Prostaglandins change cell shape and increase intercellular gap junctions in osteoblasts cultured from rat fetal calvaria
Prostaglandins change cell shape and increase intercellular gap junctions in osteoblasts cultured from rat fetal calvaria
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前列腺素改变大鼠胎儿颅盖培养的成骨细胞的细胞形状并增加细胞间间隙连接
DOI：
10.1002/jbmr.5650010302
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发表时间：
1986
影响因子：
6.2
通讯作者：
W. Peck
中科院分区：
文献类型：
--
作者：
V. Shen;L. Rifas;G. Kohler;W. Peck
越来越多的实验证据表明，成骨细胞可能是骨重建局部激活的细胞中介。为了阐明这些细胞在活化中的作用，我们研究了已知的吸收刺激剂--暴露于PGE 2和PGE 1，骨吸收的促进剂，迅速（20分钟内）转化成骨细胞从扁平到星状形状（形状变化），并显着增加细胞间（间隙）连接的外观在10分钟内。这两种效果直接相关的前列腺素浓度，低至1 nM有效。PGE 1，而不是PGB 1、PGF 1 α、PGD 2和PGF 2 α，模拟了PGE 2对形状改变的实质性作用。形状变化和间隙连接的形成似乎独立出现。甲状旁腺素，形状变化的诱导剂，并没有明显影响缝隙连接的数量。秋水仙碱（一种微管聚合抑制剂）和三氟拉嗪（一种钙调蛋白作用抑制剂）减弱了PGE 2介导的形状变化，但不减弱PGE 2对间隙连接的影响。形状变化和间隙连接形成可能是局部激活中的重要事件，表面成骨细胞的形状变化用于暴露对破骨细胞具有趋化性的骨表面，间隙连接传播局部启动的激活信息。
Mounting experimental evidence indicates that osteoblasts may be cellular intermediaries in the local activation of bone remodeling. To elucidate the role of these cells in activation, we examined the effects of prostaglandins (PGs), known resorption stimulators, on cell shape and intercellular junctional relationships in osteoblasts cultured from rat fetal calvaria. Exposure to PGE2 and PGE1, promoters of bone resorption, rapidly (within 20 min) converted the osteoblasts from a flattened to a stellate shape (shape change), and markedly increased the appearance of intercellular (gap) junctions within 10 min. Both effects were directly related to the prostaglandin concentration, as little as 1 nM being effective. PGE1, but not PGB1, PGF1α, PGD2, and PGF2α, mimicked the substantial effect of PGE2 on shape change. Shape change and gap junction formation appear to arise independently. PTH, an inducer of shape change, did not affect the number of gap junctions appreciably. Colchicine, a microtubule polymerization inhibitor, and trifluoperazine, an inhibitor of calmodulin action, blunted PGE2‐mediated shape change but not the effect of PGE2 on gap junctions. Shape change and gap junction formation may be important events in local activation, shape changes in surface osteoblasts serving to expose bone surfaces which are chemotactic for osteoclasts and gap junctions propagating locally initiated activation messages.
间隙连接结构和细胞间耦合调节：是否涉及钙调蛋白？
DOI：
--
发表时间：
1984
期刊：
Federation proceedings
影响因子：
--
作者：
Peracchia,C;Bernardini,G
通讯作者：
Bernardini,G