Bre1, an E3 ubiquitin ligase required for recruitment and substrate selection of Rad6 at a promoter
Bre1, an E3 ubiquitin ligase required for recruitment and substrate selection of Rad6 at a promoter
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DOI：
10.1016/s1097-2765(02)00802-x
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发表时间：
2003-01-01
期刊：
影响因子：
16
通讯作者：
Shilatifard, A
中科院分区：
文献类型：
--
作者：
Wood, A;Krogan, NJ;Shilatifard, A
Rad6催化组蛋白H2B泛素化是COMPASS实现组蛋白H3甲基化的必要条件。我们确定Bre1可能是Rad6转录作用的E3。Bre1含有C3HC4 （RING）指，并与Rad6在一个复合物中存在。Bre1的RING finger是组蛋白H2B泛素化、赖氨酸4和H3甲基化以及端粒沉默所必需的。染色质免疫沉淀实验表明Rad6和Bre1都被募集到一个启动子上。Bre1对Rad6的募集至关重要，并致力于Rad6的转录途径。这些结果表明Bre1可能是E3酶，指导Rad6修饰染色质并最终影响基因表达。
Ubiquitination of histone H2B catalyzed by Rad6 is required for methylation of histone H3 by COMPASS. We identified Bre1 as the probable E3 for Rad6's role in transcription. Bre1 contains a C3HC4 (RING) finger and is present with Rad6 in a complex. The RING finger of Bre1 is required for ubiquitination of histone H2B, methylation of lysine 4 and 79 of H3 and for telomeric silencing. Chromatin immunoprecipitation experiments indicated that both Rad6 and Bre1 are recruited to a promoter. Bre1 is essential for this recruitment of Rad6 and is dedicated to the transcriptional pathway of Rad6. These results suggest that Bre1 is the likely E3 enzyme that directs Rad6 to modify chromatin and ultimately to affect gene expression.