Sorting of circulating tumor cells (MV3-melanoma) and red blood cells using non-inertial lift.
Sorting of circulating tumor cells (MV3-melanoma) and red blood cells using non-inertial lift.
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DOI：
10.1063/1.4818907
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发表时间：
2013-08
期刊：
影响因子：
3.2
通讯作者：
T. M. Geislinger;T. Franke
中科院分区：
文献类型：
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作者：
T. M. Geislinger;T. Franke
我们展示了非惯性升力诱导细胞分选（NILICS）的方法，这是一种在低雷诺数流动条件下，在简单的单层微流体装置中连续、被动和无标记的细胞分选方法。在实验中，我们利用非惯性升力效应从不同红细胞比容高达9%的红细胞悬浮液中分选循环mv3 -黑色素瘤细胞。我们分析了分离过程以及红细胞压积和体积流速的影响。在Hct = 9%时，我们实现了mv3细胞的分选效率高达EMV3 = 100%，并通过重新培养分选细胞来证明细胞活力。
We demonstrate the method of non-inertial lift induced cell sorting (NILICS), a continuous, passive, and label-free cell sorting approach in a simple single layer microfluidic device at low Reynolds number flow conditions. In the experiments, we exploit the non-inertial lift effect to sort circulating MV3-melanoma cells from red blood cell suspensions at different hematocrits as high as 9%. We analyze the separation process and the influence of hematocrit and volume flow rates. We achieve sorting efficiencies for MV3-cells up to EMV3 = 100% at Hct = 9% and demonstrate cell viability by recultivation of the sorted cells.