Infection of Chinese cabbage by Plasmodiophora brassicae leads to a stimulation of plant growth:: impacts on cell wall metabolism and hormone balance
Infection of Chinese cabbage by Plasmodiophora brassicae leads to a stimulation of plant growth:: impacts on cell wall metabolism and hormone balance
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DOI：
10.1111/j.1469-8137.2004.01304.x
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发表时间：
2005-04-01
期刊：
影响因子：
9.4
通讯作者：
Prinsen, E
中科院分区：
文献类型：
--
作者：
Devos, S;Vissenberg, K;Prinsen, E
植物激素在根肿病感染中的重要性早已被认识到。在大白菜（Brassica rapa spp.）感染根腐病菌（Plasmodiophora brassicae）的早期阶段，通过定位木葡聚糖内切转葡糖基酶/水解酶（XTH）作用分析细胞扩增，并通过质谱技术筛选几类植物激素的内源浓度。感染的植物在感染早期显示出一般的短暂生长促进。此外，在感染根的表皮层中可见明显的XTH作用。观察到内源植物激素谱的复杂变化。最初的感染导致总生长素库的增加。生长素的增加，加上XTH作用的增加，导致细胞壁松弛，从而导致细胞膨胀。当第一个次级原质团形成时，感染后13天（DAI），可以被认为是植物激素代谢的转换点。21日龄疟原虫可能作为一个植物激素库，导致减少在活性细胞分裂素池和较低的吲哚-3-乙酸含量在受感染的植物。
The importance of plant hormones in clubroot infection has long been recognized. The morphological changes, such as cell division and cell elongation leading to gall formation are triggered in the early stages of infection.We analysed cell expansion by localizing Xyloglucan endoTransglucosylase/Hydrolase (XTH)-action and screened the endogenous concentrations of several classes of phytohormones by mass spectrometry in the early stages of Plasmodiophora brassicae infection in Chinese cabbage (Brassica rapa spp. pekinensis).Infected plants showed a general transient growth promotion early in infection. Furthermore a clear XTH action was visible in the epidermal layer of infected roots. Complex changes in the endogenous phytohormone profile were observed. Initially infection resulted in an increased total auxin pool.The auxin increase, together with an increased XTH action, results in wall loosening and consequently cell expansion. When the first secondary plasmodia are formed, thirteen days after infection (DAI), can be considered a switch point in phytohormone metabolism. Twenty-one DAI the plasmodia might act as a plant hormone sink resulting in a reduction in the active cytokinin pool and a lower indole-3-acetic acid content in the infected plants.