An emphysema model in rats treated intratracheally with elastase.
An emphysema model in rats treated intratracheally with elastase.
复制标题
气管内用弹性蛋白酶治疗的大鼠肺气肿模型。
DOI：
10.1016/s0013-9351(87)80199-8
复制
发表时间：
1987
影响因子：
8.3
通讯作者：
H. Kyono
中科院分区：
文献类型：
--
作者：
E. Yokoyama;Z. Nambu;I. Uchiyama;H. Kyono
为建立适合于研究肺气肿肺对大气污染物反应的肺气肿模型，对大鼠一次性气管内注射6.5单位猪胰腺弹性酶后3、7、10周的肺功能、肺组织形态和形态计量学进行了观察。与生理盐水对照组相比，弹性蛋白酶处理组大鼠的功能残气量和残气量均有不同程度的增加，但总肺活量仅在7周和10周时增加。除3周弹性酶动物外，所有动物的静态肺顺应性增加，最大流量和最大流量在用力呼气时下降，占总肺活量的50%。经弹性蛋白酶处理的小鼠肺组织表现出典型的肺气肿病变的形态改变。弹性酶处理的肺表现出平均线形截距的增加和总肺泡表面积的减少。根据这些结果，我们认为用目前剂量的弹性蛋白酶治疗7-10周的大鼠可以获得一种合适的肺气肿模型。
Pulmonary functions, morphology, and morphometry were examined in rats at 3, 7, and 10 weeks after a single intratracheal administration of 6.5 units of porcine pancreatic elastase in order to obtain a model of pulmonary emphysema which would be suitable for studying the responses of emphysematous lungs to atmospheric pollutants. Functional residual capacity and residual volume of the elastase-treated rats increased at all the times studied, but their total lung capacity increased only at 7 and 10 weeks compared with those of the saline-treated control rats. The increase in static lung compliance and the decrease in peak flow and maximum flow at 50% of total lung capacity during forced expiration were also observed in all except the 3-week elastase animals. The elastase-treated lungs showed morphological changes characteristic of emphysematous lesions. The increase in mean linear intercept length and the decrease in total alveolar surface area were demonstrated by these elastase-treated lungs. Based on these results, we conclude that an adequate and suitable model of pulmonary emphysema could be obtained in rats 7–10 weeks after treatment with the present dose of elastase.