Effects of local osteoprotegerin gene transfection on orthodontic root resorption during retention: an in vivo micro-CT analysis.
Effects of local osteoprotegerin gene transfection on orthodontic root resorption during retention: an in vivo micro-CT analysis.
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DOI：
10.1111/j.1601-6343.2011.01532.x
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发表时间：
2012-02
影响因子：
3.1
通讯作者：
N. Zhao;Yucun Liu;Hiroyuki Kanzaki;W. Liang;J. Ni;J. Lin
中科院分区：
文献类型：
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作者：
N. Zhao;Yucun Liu;Hiroyuki Kanzaki;W. Liang;J. Ni;J. Lin
目的 外牙根吸收（ERR）是正畸治疗的严重并发症。本研究的目的是评估局部护骨素 (OPG) 基因转染对保留期间 ERR 的影响。材料和方法 18 只 6 周龄雄性 Wistar 大鼠分为三组。所有大鼠均接受 2 周的正畸牙齿移动，然后进行 2 周的保留期。保留期间，三组大鼠均接受局部OPG基因转染（OPG转染组，n=6）、模拟载体转染（模拟组，n=6）或不注射（对照组，n=6）。所有三组的 ERR 在三个不同的时间点通过体内显微 CT 分析进行评估：基线、正畸牙齿移动的最后一天和保留的最后一天。结果在OPG转染组中，基线时的ERR与保留最后一天的ERR之间没有显着差异。到保留的最后一天，OPG 转染组的 ERR 修复率在统计学上高于其他组的修复率 (p<0.001)。结论结果表明，局部OPG基因转染显着增强了保留期间ERR的修复。因此，局部 OPG 基因转染可能是保留期间 ERR 修复的有用工具。
OBJECTIVES External root resorption (ERR) is a serious complication of orthodontic treatment. Aim of this study was to evaluate the effects of local osteoprotegerin (OPG) gene transfection on ERR during retention. MATERIAL AND METHODS Eighteen 6-week-old male Wistar rats were divided into three groups. All the rats were subjected to 2 weeks of orthodontic tooth movement followed by a 2-week retention period. During retention, the three groups of rats received local OPG gene transfection (OPG transfection group, n=6), mock vector transfection (mock group, n=6), or no injections (control group, n=6). ERR of all three groups was evaluated with in vivo micro-CT analysis at three different time points: baseline, the last day of orthodontic tooth movement, and the last day of retention. RESULTS In the OPG transfection group, there was no significant difference between ERR at the baseline and ERR on the last day of retention. By the last day of retention, the repair ratio of ERR in the OPG transfection group was statistically higher in relation to the repair ratio of the other groups (p<0.001). CONCLUSION The results indicated that local OPG gene transfection significantly enhanced the repair of ERR during retention. Local OPG gene transfection might therefore be a useful tool for ERR repair during retention.