Zoo-fluorescence in situ hybridization analysis of human and Indian muntjac karyotypes (Muntiacus muntjak vaginalis) reveals satellite DNA clusters at the margins of conserved syntenic segments
Zoo-fluorescence in situ hybridization analysis of human and Indian muntjac karyotypes (Muntiacus muntjak vaginalis) reveals satellite DNA clusters at the margins of conserved syntenic segments
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DOI：
10.1023/b:chro.0000032298.22346.46
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发表时间：
1997-06-01
影响因子：
2.6
通讯作者：
Scherthan, H
中科院分区：
文献类型：
--
作者：
Fronicke, L;Scherthan, H
动物荧光原位杂交(FISH)与人类全染色体特异的PINT探针进行杂交，发现印度仓鼠(2n=6，7雌性，雄性)与人类核型(2n=46)具有广泛的同源性。42个保守的同线片段，对应于除Y染色体以外的所有人类染色体，产生了对Mutjac互补的近乎完整的覆盖，并揭示了种间片段同源性的边缘。为了检验一种新的假设，即在中国仓鼠染色体臂上的一个CS-卫星探针的照射下，一个组织间卫星DNA基因座可以标记出其祖先的融合点(林冲，Sasi R，Fan YS，Chen Z-Q(1991))，这是亚洲仓鼠核型进化中串联染色体融合的新证据。Chromosoma 101：19-24)，我们将Zoo-FISH与C5卫星定位相结合。在单倍体印度鼠基因组中检测到26个间隙卫星DNA基因座，并与保守的人/印度鼠共线片段的边缘共定位。这些结果得到了双色FISH的证实，并符合印度芒果染色体的串联融合假说。此外，在猪、牛和印度鸣禽动物园-鱼类图谱中发现的保守的同线片段组合揭示了祖先的偶指龙染色体。
Zoo-fluorescence in situ hybridization (FISH) with human whole chromosome-specific paint probes revealed extensive homoeologies between Indian muntjac (2n=6, 7 female, male) and human karyotypes (2n=46). Forty-two conserved syntenic segments, corresponding to all human chromosomes except the Y chromosome, produced a near-complete coverage of the muntjac complement and revealed margins of interspecific segmental homoeology. To test the hypothesis that interstitial satellite DNA loci, illuminated by a Chinese muntjac CS-satellite probe in Indian muntjac chromosome arms, mark ancestral fusion points (Lin CC, Sasi R, Fan YS, Chen Z-Q (1991) New evidence for tandem chromosome fusions in the karyotypic evolution of the Asian muntjacs. Chromosoma 101: 19-24), we combined Zoo-FISH with C5 satellite mapping. Twenty-six interstitial satellite DNA loci were detected in the haploid Indian muntjac genome and were found to co-localize with the margins of conserved human/Indian muntjac syntenic segments. These results were confirmed by two-colour FISH and are in accordance with the tandem fusion hypothesis for Indian muntjac chromosomes. Furthermore, conserved syntenic segment combinations detected in pig, cattle and Indian muntjac Zoo-FISH maps reveal ancestral artiodactyl chromosomes.