Preparation of an rhBMP-2 loaded mesoporous bioactive glass/calcium phosphate cement porous composite scaffold for rapid bone tissue regeneration
Preparation of an rhBMP-2 loaded mesoporous bioactive glass/calcium phosphate cement porous composite scaffold for rapid bone tissue regeneration
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负载rhBMP-2的介孔生物活性玻璃/磷酸钙水泥多孔复合支架的制备用于骨组织快速再生
DOI：
10.1039/c5tb01423a
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发表时间：
2015-01-01
影响因子：
7
通讯作者：
Li, Yongsheng
中科院分区：
文献类型：
--
作者：
Li, Nan;Jiang, Chuan;Li, Yongsheng
本研究采用离心包埋法将介孔生物活性玻璃（MBG）与磷酸钙骨水泥（CPC）材料复合，构建了一种新型复合支架。此外，重组人骨形态发生蛋白-2（rhBMP-2）通过冷冻干燥过程容易地并入该支架中。结果表明，所制备的支架不仅具有分级的孔结构（约200 mm和2-10 μ m的互连孔）和足够的抗压强度（高达1.4 MPa），而且具有优异的药物释放性能，rhBMP-2的持续释放超过7 d。为了评价rhBMP-2负载的MBG/CPC支架的成骨能力，进行了与骨髓基质细胞（BMSCs）的体外细胞培养。值得注意的是，这种复合支架对BMSCs的增殖和成骨分化具有有利的影响。此外，使用兔桡骨缺损模型进行体内骨组织再生。结果表明，rhBMP-2的掺入可以诱导成骨效率的显着提高，特别是在早期阶段。此外，rhBMP-2负载的MBG/CPC支架中获得更好的生物降解性相比，其他。因此，rhBMP-2负载的MBG/CPC支架材料在快速骨组织再生领域具有巨大的应用潜力。
In this work, a novel composite scaffold was constructed by combining mesoporous bioactive glass (MBG) and calcium phosphate cement (CPC) materials using a simple centrifugal embedding approach. Furthermore, recombinant human bone morphogenetic protein-2 (rhBMP-2) was facilely incorporated into this scaffold through a freeze-drying process. It is found that the resultant scaffold not only presents a hierarchical pore structure (interconnected pores of around 200 mm and 2-10 mu m) and a sufficient compressive strength (up to 1.4 MPa), but also exhibits excellent drug delivery properties, presenting sustained release of rhBMP-2 for over 7 d. In order to evaluate the osteogenetic capacity of the rhBMP-2 loaded MBG/CPC scaffold, in vitro cell culture with bone marrow stromal cells (BMSCs) was conducted. Notably, this composite scaffold presents a favorable effect on the proliferation and osteogenetic differentiation of BMSCs. Furthermore, in vivo bone tissue regeneration was conducted using a rabbit radius defect model. It is demonstrated that the incorporation of rhBMP-2 can induce a significant improvement of osteogenetic efficiency, especially in the early stage. Moreover, better biodegradability was obtained in the rhBMP-2 loaded MBG/CPC scaffold compared to the others. Therefore, it is anticipated that the rhBMP-2 loaded MBG/CPC scaffold is of great potential in the field of rapid bone tissue regeneration.