Acute phase response in lame crossbred dairy cattle.
Acute phase response in lame crossbred dairy cattle.
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DOI：
10.14202/vetworld.2016.1204-1208
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发表时间：
2016-11
期刊：
影响因子：
1.6
通讯作者：
Bansal BK
中科院分区：
文献类型：
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作者：
Bagga A;Randhawa SS;Sharma S;Bansal BK
本研究旨在研究基于急性时相蛋白（APP）如C-反应蛋白（CRP）、结合珠蛋白（Hp）、血清淀粉样蛋白A（SAA）和纤维蛋白原的跛脚杂交奶牛的急性时相反应。从卢迪亚纳东南部的一个当地奶牛场选择跛足动物（n=30），在农场兽医注意到跛足后3-7天内选择，为期6个月，与非跛足健康奶牛（n=10）的泌乳阶段成比例分层。所有奶牛在其他方面都很健康，没有任何其他炎症问题，如肺炎、肠炎、乳腺炎或任何类型的急性子宫炎症。从所有动物中采集血样;分离血清和血浆样品并储存在−20°C下。CRP、Hp、SAA采用夹心ELISA法测定，纤维蛋白原采用热沉淀法测定。跛行奶牛中的SAA水平显著较高（22.19±0.85 µg/ml），约为非跛行奶牛（8.89±0.72 µg/ml）的3倍，而跛行奶牛中的血清Hp浓度（21.71±3.32 mg/dl）约为非跛行奶牛（1.17±0.07 mg/dl）的20倍。纤维蛋白原在跛行牛中也增加（3.97±0.22 g/L），与非跛行组（1.40±0.17 g/L）相比。本研究首次分析了跛行牛的血清CRP水平，跛行牛的血清CRP水平（4.41±0.33 mg/L）显著高于非跛行牛（0.61±0.14 mg/L）。与非跛足牛相比，跛足牛具有更多的足底肿胀、足底溃疡和白色线病变。可以得出结论，与非跛行牛相比，跛行牛表现出高水平的APP，包括CRP、Hp、SAA和纤维蛋白原。
The study was undertaken to study acute phase response based on acute phase proteins (APPs) such as C-reactive protein (CRP), haptoglobin (Hp), serum amyloid A (SAA), and fibrinogen in lame crossbred dairy cattle. Lame animals (n=30) were selected within 3-7 days of being noticed as lame by the farm veterinarian, from a local dairy farm in southeast Ludhiana over a period of 6 months, stratified proportionately with respect to stage of lactation with non-lame healthy cows (n=10). All the cows were otherwise healthy and did not have any other inflammatory problems such as pneumonia, enteritis, mastitis, or any kind of acute uterine inflammation. Blood samples were collected from all the animals; serum and plasma samples were separated and stored at −20°C. The levels of CRP, Hp, and SAA were estimated using Sandwich ELISA, whereas fibrinogen was estimated by heat precipitation method. SAA levels in lame cows were significantly higher (22.19±0.85 µg/ml), approximately 3 times as compared to non-lame cows (8.89±0.72 µg/ml), whereas serum Hp concentration was approximately 20 times higher in the lame cattle (21.71±3.32 mg/dl) as compared to non-lame cows (1.17±0.07 mg/dl). Fibrinogen also increased in the lame cattle (3.97±0.22 g/L) as compared to non-lame group (1.40±0.17 g/L). Serum CRP levels analyzed in the lame cattle for the first time in the present study, and significant high concentration was appreciated in lame cattle (4.41±0.33 mg/L) as compared to non-lame cattle (0.61±0.14 mg/L). Lame cattle were having more of sole hemorrhages, sole ulcers, and white line lesions as compared to non-lame cattle. It can be concluded that lame cattle exhibit high levels of APPs including CRP, Hp, SAA, and fibrinogen as compared to non-lame cattle.