Suppression of a single BAHD gene in Setaria viridis causes large, stable decreases in cell wall feruloylation and increases biomass digestibility.
Suppression of a single BAHD gene in Setaria viridis causes large, stable decreases in cell wall feruloylation and increases biomass digestibility.
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DOI：
10.1111/nph.14970
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发表时间：
2018-04
期刊：
影响因子：
--
通讯作者：
Molinari HBC
中科院分区：
文献类型：
--
作者：
de Souza WR;Martins PK;Freeman J;Pellny TK;Michaelson LV;Sampaio BL;Vinecky F;Ribeiro AP;da Cunha BADB;Kobayashi AK;de Oliveira PA;Campanha RB;Pacheco TF;Martarello DCI;Marchiosi R;Ferrarese-Filho O;Dos Santos WD;Tramontina R;Squina FM;Centeno DC;Gaspar M;Braga MR;Tiné MAS;Ralph J;Mitchell RAC;Molinari HBC
植物细胞壁中阿拉伯木聚糖（AX）的阿魏酰化是植物抵抗酶攻击的关键决定因素，使其成为改良禾本科作物的目标，并在禾本科植物进化中引起人们的兴趣。缺乏有关基因的可靠证据，因此我们研究了我们在BAHD酰基辅酶A转移酶家族中鉴定的候选基因。我们在模式草狗尾草（SvBAHD 01）和二穗短柄草（BdBAHD 01）中使用直向同源物的RNA干扰（RNAi）沉默，并确定对AX阿魏酰化的影响。在狗尾草属中沉默SvBAHD 01导致c.在四代中，茎中AX阿魏酸化一致降低60%。BdBAHD 01在短柄草茎中的沉默降低的阿魏酸化少得多，可能是由于功能冗余基因的更高表达。狗尾草SvBAHD 01 RNAi植物显示：总木质素没有减少，被对香豆酸酰化的阿拉伯糖大约加倍，未分级细胞壁的二维NMR光谱的变化与生物化学估计一致，对总生物质产量没有影响，生物质糖化效率增加40- 60%。我们提供了BAHD 01基因在AX阿魏酰化中的关键作用的第一个强有力的证据，并证明它是一个有前途的目标，为生物燃料，生物精炼和动物营养应用的禾本科作物的改进。另见Gómez & McQueen‐Mason对这篇文章的评论，218：5-7。
Feruloylation of arabinoxylan (AX) in grass cell walls is a key determinant of recalcitrance to enzyme attack, making it a target for improvement of grass crops, and of interest in grass evolution. Definitive evidence on the genes responsible is lacking so we studied a candidate gene that we identified within the BAHD acyl‐CoA transferase family. We used RNA interference (RNAi) silencing of orthologs in the model grasses Setaria viridis (SvBAHD01) and Brachypodium distachyon (BdBAHD01) and determined effects on AX feruloylation. Silencing of SvBAHD01 in Setaria resulted in a c. 60% decrease in AX feruloylation in stems consistently across four generations. Silencing of BdBAHD01 in Brachypodium stems decreased feruloylation much less, possibly due to higher expression of functionally redundant genes. Setaria SvBAHD01 RNAi plants showed: no decrease in total lignin, approximately doubled arabinose acylated by p‐coumarate, changes in two‐dimensional NMR spectra of unfractionated cell walls consistent with biochemical estimates, no effect on total biomass production and an increase in biomass saccharification efficiency of 40–60%. We provide the first strong evidence for a key role of the BAHD01 gene in AX feruloylation and demonstrate that it is a promising target for improvement of grass crops for biofuel, biorefining and animal nutrition applications. See also the Commentary on this article by Gómez & McQueen‐Mason, 218: 5–7.
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DOI：
10.1111/tpj.12510
发表时间：
2014-06
期刊：
The Plant journal : for cell and molecular biology
影响因子：
--
作者：
Marita JM;Hatfield RD;Rancour DM;Frost KE
通讯作者：
Frost KE
DOI：
10.1073/pnas.1202079109
发表时间：
2012-10-16
影响因子：
11.1
作者：
Chiniquy, Dawn;Sharma, Vaishali;Ronald, Pamela C.
通讯作者：
Ronald, Pamela C.
影响因子：
3.2
作者：
Casler, MD;Jung, HJG
通讯作者：
Jung, HJG
影响因子：
46.9
作者：
Bennetzen, Jeffrey L.;Schmutz, Jeremy;Devos, Katrien M.
通讯作者：
Devos, Katrien M.
影响因子：
7.2
作者：
d'Yvoire, Madeleine Bouvier;Bouchabke-Coussa, Oumaya;Sibout, Richard
通讯作者：
Sibout, Richard