An in Vitro Model for the Study of Human Hair Growth
An in Vitro Model for the Study of Human Hair Growth
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DOI：
10.1111/j.1749-6632.1991.tb24386.x
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发表时间：
1991-12
影响因子：
5.2
通讯作者：
M. Philpott;G. Westgate;T. Kealey
中科院分区：
文献类型：
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作者：
M. Philpott;G. Westgate;T. Kealey
用显微切割法从人头皮皮肤中分离出生长期毛囊。毛囊的分离是通过使用手术刀切割皮肤-皮下脂肪交界处的毛囊来实现的。然后使用手表制造商的钳子从脂肪中取出完整的毛囊。在24孔多孔板的单个孔中，分离毛囊在添加Williams E的培养液中保持自由漂浮，4天后毛囊长度显著增加。长度的增加被认为是由于角化毛干的产生，而与毛囊形态的丧失无关。[甲基-~3H]胸腺嘧啶核苷放射自显影证实，在体外，DNA合成的模式保持不变；此外，角蛋白的[35S]蛋氨酸标记表明，它们的合成模式不随维持而改变。血清对体外培养的毛囊生长有抑制作用，在无血清培养条件下，毛囊生长可达10天，提示体外培养的毛囊能够通过分泌相关生长因子来调节自身的生长。这可能有助于确定毛囊中的自分泌/旁分泌机制。这一模型对毛囊生物学的重要性进一步得到证明，观察到转化生长因子-β1在体外对毛囊具有负的生长调节作用，并且EGF及其其他受体配体转化生长因子-α在体内模拟了EGF对绵羊和小鼠的脱毛作用。
Human anagen hair follicles were isolated by microdissection from human scalp skin. Isolation of the hair follicles was achieved by cutting the follicle at the dermosubcutaneous fat interface using a scalpel blade. Intact hair follicles were then removed from the fat using watch makers' forceps. Isolated hair follicles maintained free floating in supplemented Williams E medium in individual wells of 24-well multiwell plates showed a significant increase in length over 4 days. The increase in length was seen to be attributed to the production of a keratinized hair shaft, and was not associated with the loss of hair follicle morphology. [Methyl-3H]thymidine autoradiography confirmed that in vitro the in vivo pattern of DNA synthesis was maintained; furthermore, [35S]methionine labeling of keratins showed that their patterns of synthesis did not change with maintenance. Serum was found to inhibit hair follicle growth in vitro; and when follicles were maintained in serum-free medium, they grew for up to 10 days, suggesting that in vitro the hair follicles are able to regulate their own growth, possibly by the production of relevant growth factors. This may prove useful in identifying the autocrine/paracrine mechanisms that operate in the hair follicle. The importance of this model to hair follicle biology is further demonstrated by the observations that TGF-beta 1 has a negative growth regulatory effect on hair follicles in vitro and that EGF and its other receptor ligand TGF-alpha mimic the in vivo depilatory effects of EGF that have been reported for sheep and mice.