Hemopexin as biomarkers for analyzing the biological responses associated with exposure to silica nanoparticles.
Hemopexin as biomarkers for analyzing the biological responses associated with exposure to silica nanoparticles.
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DOI：
10.1186/1556-276x-7-555
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发表时间：
2012-10-08
影响因子：
--
通讯作者：
Tsutsumi Y
中科院分区：
文献类型：
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作者：
Higashisaka K;Yoshioka Y;Yamashita K;Morishita Y;Pan H;Ogura T;Nagano T;Kunieda A;Nagano K;Abe Y;Kamada H;Tsunoda S;Nabeshi H;Yoshikawa T;Tsutsumi Y
纳米材料的实际应用正在迅速扩展到各种领域。然而，纳米材料的潜在有害影响引发了人们对其安全性的担忧。因此，开发一种风险评估系统，以便对纳米材料的安全性进行评估或预测，是非常重要的。在这里，我们试图通过使用二维差示凝胶电泳(2D-DGE)分析对血浆蛋白的全面筛选来识别纳米材料引起的健康影响的新的生物标记物。最初，我们使用2D-DGE分析了尾静脉注射0.8 mg/只直径为70 nm(NSP70)的二氧化硅纳米颗粒(NSP70)或生理盐水后小鼠血浆蛋白水平的变化。通过定量图像分析，发现了代表表达变化2.0倍的蛋白质点，并用质谱仪进行了鉴定。在这些蛋白质中，我们将重点放在血凝蛋白作为一个潜在的生物标志物上。血浆中的血凝素水平随着二氧化硅颗粒尺寸的减小而升高。另外，血凝素的产生依赖于纳米材料的特性。这些结果表明，血凝素可能是一个额外的生物标志物，用于分析与接触二氧化硅纳米颗粒相关的生物反应。我们相信，这项研究将有助于生物标志物的开发，以确保二氧化硅纳米颗粒的安全性。
Practical uses of nanomaterials are rapidly spreading to a wide variety of fields. However, potential harmful effects of nanomaterials are raising concerns about their safety. Therefore, it is important that a risk assessment system is developed so that the safety of nanomaterials can be evaluated or predicted. Here, we attempted to identify novel biomarkers of nanomaterial-induced health effects by a comprehensive screen of plasma proteins using two-dimensional differential in gel electrophoresis (2D-DIGE) analysis. Initially, we used 2D-DIGE to analyze changes in the level of plasma proteins in mice after intravenous injection via tail veins of 0.8 mg/mouse silica nanoparticles with diameters of 70 nm (nSP70) or saline as controls. By quantitative image analysis, protein spots representing >2.0-fold alteration in expression were found and identified by mass spectrometry. Among these proteins, we focused on hemopexin as a potential biomarker. The levels of hemopexin in the plasma increased as the silica particle size decreased. In addition, the production of hemopexin depended on the characteristics of the nanomaterials. These results suggested that hemopexin could be an additional biomarker for analyzing the biological responses associated with exposure to silica nanoparticles. We believe that this study will contribute to the development of biomarkers to ensure the safety of silica nanoparticles.