Abiotic surface sensing and biofilm-dependent regulation of gene expression in Escherichia coli
Abiotic surface sensing and biofilm-dependent regulation of gene expression in Escherichia coli
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DOI：
10.1128/jb.181.19.5993-6002.1999
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发表时间：
1999-10-01
影响因子：
3.2
通讯作者：
Lejeune, P
中科院分区：
文献类型：
--
作者：
Prigent-Combaret, C;Vidal, O;Lejeune, P
为了得到更多的信息，细菌表面传感和生物膜依赖性调控的基因表达在大肠杆菌K-12，随机插入诱变与Mu dX，一个迷你Mu携带启动子的lacZ基因，进行ompR 234贴壁菌株，和一个简单的屏幕开发，以评估基因表达的变化，在生物膜细胞与非生物膜细胞。该筛选揭示了基因表达模式的主要变化发生在生物膜发育期间：38%的基因的转录在生物膜内受到影响，不同的细胞功能在固着细菌中更多地表达：OmpC孔蛋白，甘氨酸甜菜碱的高亲和力转运系统（由proU操纵子编码），colanic酸胞外多糖（wca基因座，以前称为cps）、三肽酶T（pepT）和镍高亲和力转运系统（nikA）。另一方面，在生物膜中鞭毛蛋白（fliC）和推定的92个氨基酸的蛋白质（f92）的合成都减少。生物膜中基因表达的这种遗传重编程似乎是多种环境物理化学条件变化的结果。在这项工作中，我们表明，生物膜内的细菌遇到较高的渗透压条件下，更大的氧气限制，和更高的细胞密度比在液相中。
To get further information on bacterial surface sensing and biofilm-dependent regulation of gene expression in Escherichia coli K-12, random insertion mutagenesis with Mu dX, a mini-Mu carrying the promoterless lacZ gene, was performed with an ompR234 adherent strain, and a simple screen was developed to assess changes in gene expression in biofilm cells versus planktonic cells. This screen revealed that major changes in the pattern of gene expression occur during biofilm development: the transcription of 38% of the genes was affected within biofilms, Different cell functions were more expressed in sessile bacteria: the OmpC porin, the high-affinity transport system of glycine betaine (encoded by the proU operon), the colanic acid exopolysaccharide (wca locus, formerly called cps), tripeptidase T (pepT), and the nickel high-affinity transport system (nikA), On the other hand, the syntheses of flagellin (fliC) and of a putative protein of 92 amino acids (f92) were both reduced in biofilms. Such a genetic reprogramming of gene expression in biofilms seems to result from changes in multiple environmental physicochemical conditions. In this work, we show that bacteria within biofilms encounter higher-osmolarity conditions, greater oxygen limitation, and higher cell density than in the liquid phase.