Critical role of NK1+ T cells in IL-12-induced immune responses in vivo.
Critical role of NK1+ T cells in IL-12-induced immune responses in vivo.
复制标题
NK1 T 细胞在 IL-12 诱导的体内免疫反应中发挥关键作用。
作者：
T. Kawamura;K. Takeda;S. K. Mendiratta;H. Kawamura;L. Van Kaer;H. Yagita;T. Abo;K. Okumura
CD1 依赖性 NK1+ T 细胞在通过 TCR 刺激后迅速产生 IL-4。因此，这些细胞可能在 Th2 反应的启动中发挥重要作用。在这里，我们证明 NK1+ T 细胞组成型表达 IL-12 和 IFN-γ 受体，并且 IL-12 诱导这些细胞中穿孔素的产生。此外，虽然 IL-12 诱导高水平的 IFN-γ 以及肝或脾单核细胞针对肿瘤细胞的细胞毒活性，但在 NK1+ T 细胞发育受损的 CD1 缺陷小鼠中，IL-12 的这种作用显着降低。这些结果表明，NK1+ T 细胞在 IL-12 诱导的 IFN-γ 产生以启动 Th1 免疫反应以及作为 IL-12 诱导的细胞毒性效应细胞启动抗肿瘤免疫中发挥着关键作用。
CD1-dependent NK1+ T cells rapidly produce IL-4 upon stimulation through the TCR. These cells may therefore play an important role in the initiation of Th2 responses. Here, we show that NK1+ T cells constitutively express receptors for IL-12 and IFN-gamma, and that IL-12 induces production of perforin in these cells. Moreover, while IL-12 induces high levels of IFN-gamma and cytotoxic activity of hepatic or splenic mononuclear cells against tumor cells, this effect of IL-12 is significantly reduced in CD1-deficient mice with impaired NK1+ T cells development. These results indicate that NK1+ T cells play a critical role in IL-12-induced production of IFN-gamma to initiate Th1 immune responses and as IL-12-induced cytotoxic effector cells to initiate antitumor immunity.