Current covalent modification methods for detecting RNA in fixed and living cells
Current covalent modification methods for detecting RNA in fixed and living cells
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DOI：
10.1016/j.ymeth.2015.11.016
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发表时间：
2016-04-01
期刊：
影响因子：
4.8
通讯作者：
Rentmeister, Andrea
中科院分区：
文献类型：
--
作者：
Holstein, Josephin M.;Rentmeister, Andrea
标记RNA对于阐明特定转录物的定位、运输和调节（理想地在活细胞中）特别感兴趣。已经开发了许多方法，从杂交探针到遗传编码的报告基因和化学-酶促方法。这篇评论的重点是共价标记的方法，依赖于引入一个小的反应基团到新生的或完成的转录后，生物正交点击化学。国家的固定和活细胞中的RNA标记的方法将被提出和新兴的战略，在复杂的细胞环境中具有巨大的应用潜力将被讨论。(C)2015爱思唯尔公司All rights reserved.
Labeling RNAs is of particular interest for elucidating localization, transport, and regulation of specific transcripts, ideally in living cells. Numerous methods have been developed ranging from hybridizing probes to genetically encoded reporters and chemo-enzymatic approaches. This review focuses on covalent labeling approaches that rely on the introduction of a small reactive group into the nascent or completed transcript followed by bioorthogonal click chemistry. State of the approaches for labeling RNA in fixed and living cells will be presented and emerging strategies with great potential for application in the complex cellular environment will be discussed. (C) 2015 Elsevier Inc. All rights reserved.