HISTOCOMPATIBILITY ANTIGENS OF HUMAN PLASMA - LOCALIZATION TO HLD-3 LIPOPROTEIN FRACTION
HISTOCOMPATIBILITY ANTIGENS OF HUMAN PLASMA - LOCALIZATION TO HLD-3 LIPOPROTEIN FRACTION
复制标题
DOI：
10.1097/00007890-197309000-00007
复制
发表时间：
1973-01-01
期刊：
影响因子：
6.2
通讯作者：
RODEY, GE
中科院分区：
文献类型：
--
作者：
ASTER, RH;MISKOVICH, BH;RODEY, GE
用羟基磷灰石层析法和密度梯度超离心法从正常血浆中分离脂蛋白组分，测定组织相容性抗原HL-A2和HL-A7。两种抗原仅在含有具有[α]电泳迁移率的HDL-3亚类脂蛋白的级分中回收。在正常人血液中，可溶性HL-A2抗原约占总HL-A2活性的14%，而总活性的73%、7%和6%分别与血小板、粒细胞和淋巴细胞结合。当在来自HL-A2阴性受试者的血浆中温育来自HL-A2阳性供体的血小板时，在血浆中回收具有HL-A2活性的HDL-3脂蛋白，显然已经从血小板膜释放。这些数据表明，可溶性组织相容性抗原的人血浆漂浮在超离心和沉淀的羟基磷灰石与HDL-3脂蛋白。它们最初可能作为细胞膜成分合成，然后释放到全身循环中。这些研究结果移植免疫的可能影响进行了讨论。
Assays for histocompatibility antigens HL-A2 and HL-A7 were performed on lipoprotein fractions isolated from normal plasma by hydroxylapatite chromatography and by density gradient ultracentrifugation. Both antigens were recovered solely in fractions containing lipoproteins of the HDL-3 subclass having [alpha] electrophoretic mobility. Soluble HL-A2 antigens were found to constitute approximately 14% of total HL-A2 activity in normal human blood, while 73, 7, and 6% of the total was bound to platelets, granulocytes, and lymphocytes, respectively. When platelets from HL-A2-positive donors were incubated with gentle agitation in plasma from HL-A2-negative subjects, HDL-3 lipoproteins having HL-A2 activity were recovered in the plasma, apparently having been released from platelet membranes. These data indicate that soluble histocompatibility antigens of human plasma float in the ultracentrifuge and elute from hydroxylapatite with HDL-3 lipoproteins. They may be synthesized originally as cell membrane components and then released into the general circulation. The possible implications of these findings for transplantation immunity are discussed.