Array tomography: A new tool for Imaging the molecular architecture and ultrastructure of neural circuits
Array tomography: A new tool for Imaging the molecular architecture and ultrastructure of neural circuits
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DOI：
10.1016/j.neuron.2007.06.014
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发表时间：
2007-07-05
期刊：
影响因子：
16.2
通讯作者：
Smith, Stephen J.
中科院分区：
文献类型：
--
作者：
Micheva, Kristina D.;Smith, Stephen J.
许多生物学功能严重依赖于组织分子结构的精细细节，而这些精细细节已经阻碍了现有成像技术的探索。这对于神经系统组织尤其如此，其中信息处理功能取决于复杂的电路和突触结构。在这里，我们描述了一种新的成像方法，称为阵列层析成像，它结合并扩展了现代光学荧光和电子显微镜方法的最高级功能。基于构建和重复染色和成像有序阵列的方法，树脂包埋的连续切片在玻璃显微镜载玻片上，阵列断层扫描允许定量，高分辨率，大视场体积成像的大量抗原，荧光蛋白和超微结构在个别组织标本。与共聚焦显微镜相比，阵列层析成像提供了更好的空间分辨率的优势，特别是沿沿着z轴，以及深度无关的免疫荧光染色。阵列断层扫描的应用可以揭示重要的，但以前看不见的大脑分子结构的特点。
Many biological functions depend critically upon fine details of tissue molecular architecture that have resisted exploration by existing imaging techniques. This is particularly true for nervous system tissues, where information processing function depends on intricate circuit and synaptic architectures. Here, we describe a new imaging method, called array tomography, which combines and extends superlative features of modern optical fluorescence and electron microscopy methods. Based on methods for constructing and repeatedly staining and imaging ordered arrays of ultrathin (50-200 nm), resin-embedded serial sections on glass microscope slides, array tomography allows for quantitative, high-resolution, large-field volumetric imaging of large numbers of antigens, fluorescent proteins, and ultrastructure in individual tissue specimens. Compared to confocal microscopy, array tomography offers the advantage of better spatial resolution, in particular along the z axis, as well as depth-independent immunofluorescent staining. The application of array tomography can reveal important but previously unseen features of brain molecular architecture.