The TARGET System: Rapid Identification of Direct Targets of Transcription Factors by Gene Regulation in Plant Cells.
The TARGET System: Rapid Identification of Direct Targets of Transcription Factors by Gene Regulation in Plant Cells.
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TARGET 系统：通过植物细胞中的基因调控快速识别转录因子的直接靶标。
DOI：
10.1007/978-1-0716-2815-7_1
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发表时间：
2023
期刊：
影响因子：
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通讯作者：
Bargmann,BastiaanOR
中科院分区：
文献类型：
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作者：
Brooks,MatthewD;Reed,KelseyM;Krouk,Gabriel;Coruzzi,GloriaM;Bargmann,BastiaanOR
TARGET系统允许快速鉴定转录因子（TF）的直接调节基因靶标。它采用植物原生质体的瞬时转化，TF的诱导核进入和随后的转录组学和/或ChIP-seq分析。与转基因的产生相比，将直接TF-靶基因调控相互作用与间接下游响应分开的能力以及进行测定所需的显著更短的时间量使得这种基于植物细胞的方法成为用于更高通量方法的有价值的工具，以鉴定多种TF的全基因组靶标，从而在植物中构建经验证的转录网络。在这里，我们描述了使用的目标系统inopsismilk根原生质体映射的基因调控网络下游的转录因子的利益。
The TARGET system allows for the rapid identification of direct regulated gene targets of transcription factors (TFs). It employs the transient transformation of plant protoplasts with inducible nuclear entry of the TF and subsequent transcriptomic and/or ChIP-seq analysis. The ability to separate direct TF–target gene regulatory interactions from indirect downstream responses and the significantly shorter amount of time required to perform the assay, compared to the generation of transgenics, make this plant cell-based approach a valuable tool for a higher throughput approach to identify the genome-wide targets of multiple TFs, to build validated transcriptional networks in plants. Here, we describe the use of the TARGET system inArabidopsisseedling root protoplasts to map the gene regulatory network downstream of transcription factors-of-interest.