An open-source control system for in vivo fluorescence measurements from deep-brain structures.
An open-source control system for in vivo fluorescence measurements from deep-brain structures.
复制标题
用于来自深部脑结构的体内荧光测量的开源控制系统。
DOI：
10.1016/j.jneumeth.2018.10.022
复制
发表时间：
2019
影响因子：
3
通讯作者：
Kreitzer,AnatolC
中科院分区：
文献类型：
--
作者：
Owen,ScottF;Kreitzer,AnatolC
通过长期植入光纤的颅内光度测量是一种广泛采用的技术，用于测量来自脑深部结构中的荧光探针的信号。最近，钙和其他神经调质的明亮、光稳定和特异性遗传编码荧光报告基因的激增极大地增加了这种技术的实用性和普及性。用于控制颅内光度测量系统中的光学组件并处理所产生的信号的基于微处理器的解决方案。该系统支持自由活动小鼠背侧纹状体的高质量颅内光度测定记录。单个系统支持在两个独立的颜色通道中同时进行荧光测量，但如果需要额外的荧光通道，则可以将多个系统集成在一起。该系统被设计为与市售或定制的光学组件结合使用。零部件的购买成本不到市售替代品的十分之一，对于没有经验的用户来说，完整的组装时间不到一天。与现有方法的比较目前可用的硬件采用各种商业、定制或混合元件，用于光学和电子元件。许多这些硬件系统要么是专门的和不灵活的，或过度设计和expensioned.ConclusionsThis开源系统增加了实验的灵活性，同时降低成本相对于目前的商用组件。所有软件和固件都是开源和可定制的，提供了一定程度的实验灵活性，这是目前商业系统所不具备的。
BackgroundIntracranial photometry through chronically implanted optical fibers is a widely adopted technique for measuring signals from fluorescent probes in deep-brain structures. The recent proliferation of bright, photo-stable, and specific genetically encoded fluorescent reporters for calcium and for other neuromodulators has greatly increased the utility and popularity of this technique.New methodHere we describe an open-source, cost-effective, microcontroller-based solution for controlling optical components in an intracranial photometry system and processing the resulting signal.ResultsWe show proof-of-principle that this system supports high quality intracranial photometry recordings from dorsal striatum in freely moving mice. A single system supports simultaneous fluorescence measurements in two independent color channels, but multiple systems can be integrated together if additional fluorescence channels are required. This system is designed to work in combination with either commercially available or custom-built optical components. Parts can be purchased for less than one tenth the cost of commercially available alternatives and complete assembly takes less than one day for an inexperienced user.Comparison with existing method(s)Currently available hardware draws on a variety of commercial, custom-built, or hybrid elements for both optical and electronic components. Many of these hardware systems are either specialized and inflexible, or over-engineered and expensive.ConclusionsThis open-source system increases experimental flexibility while reducing cost relative to current commercially available components. All software and firmware are open-source and customizable, affording a degree of experimental flexibility that is not available in current commercial systems.