Wide-field quantitative imaging of tissue microstructure using sub-diffuse spatial frequency domain imaging.
Wide-field quantitative imaging of tissue microstructure using sub-diffuse spatial frequency domain imaging.
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DOI：
10.1364/optica.3.000613
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发表时间：
2016-06-20
期刊：
影响因子：
10.4
通讯作者：
Kanick SC
中科院分区：
文献类型：
--
作者：
McClatchy DM 3rd;Rizzo EJ;Wells WA;Cheney PP;Hwang JC;Paulsen KD;Pogue BW;Kanick SC
生物组织中散射的局部测量提供了对微观结构形态的灵敏度，但对宽场应用（诸如手术引导）具有有限的效用。这项研究介绍了亚扩散空间频域成像（sd-SFDI），它使用高空间频率照明，以实现宽场采样的局部反射率。基于模型的反演恢复约化散射系数和相函数后向散射参数（γ）的宏观变化。在光学幻影测量显示定量成像的用户调谐相函数为基础的对比度与精确的去耦参数，定义的密度和尺寸尺度分布的散射体。新鲜的离体乳腺组织样本的测量结果显示，第一次，独特的聚类不同的组织类型的子扩散散射特性。研究结果支持sd-SFDI提供了无法通过扩散宽场成像获得的微观结构生物标志物的地图，并表征了基于点的光学采样无法解决的空间变化。
Localized measurements of scattering in biological tissue provide sensitivity to microstructural morphology but have limited utility to wide-field applications, such as surgical guidance. This study introduces sub-diffusive spatial frequency domain imaging (sd-SFDI), which uses high spatial frequency illumination to achieve wide-field sampling of localized reflectances. Model-based inversion recovers macroscopic variations in the reduced scattering coefficient and the phase function backscatter parameter (γ). Measurements in optical phantoms show quantitative imaging of user-tuned phase-function-based contrast with accurate decoupling of parameters that define both the density and the size-scale distribution of scatterers. Measurements of fresh ex vivo breast tissue samples revealed, for the first time, unique clustering of sub-diffusive scattering properties for different tissue types. The results support that sd-SFDI provides maps of microscopic structural biomarkers that cannot be obtained with diffuse wide-field imaging and characterizes spatial variations not resolved by point-based optical sampling.