MULLER CELLS OF CHICKEN RETINA SYNTHESIZE 11-CIS-RETINOL
MULLER CELLS OF CHICKEN RETINA SYNTHESIZE 11-CIS-RETINOL
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DOI：
10.1042/bj2850907
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发表时间：
1992-08-01
影响因子：
4.1
通讯作者：
GOURAS, P
中科院分区：
文献类型：
--
作者：
DAS, SR;BHARDWAJ, N;GOURAS, P
内源性视黄酸棕榈酸酯、视黄醇和视黄醛在以锥形视网膜(鸡)、视杆状视网膜(大鼠)和混合视网膜(猫、人)为主的神经视网膜和视网膜色素上皮(RPE)中进行了测定。视黄酸棕榈酸酯和视黄醇的11-顺式和全反式异构体在神经视网膜和视网膜色素上皮中的比例随着物种从大鼠到鸡的增加而逐渐增加。鸡肉和牛RPE的膜组分都能将全反式视黄醇酶促异构化为11-顺式视黄醇。鸡神经视网膜膜由全反式视黄醇酶促生成11-顺式视黄醇和全反式视黄醇棕榈酸酯。光镜和电子显微镜观察未见RPE对鸡神经视网膜的污染。分离、培养鸡视网膜Muller细胞，进行细胞视黄醛结合蛋白免疫细胞化学定位鉴定。培养的鸡Muller细胞从全反式视黄醇中生成全反式视黄醇棕榈酸酯、11-顺式视黄醇和11-顺式视黄醇棕榈酸酯，并将大部分11-顺式视黄醇释放到培养液中。结果表明，鸡的神经视网膜和Muller细胞特别是从全反式视黄醇合成11-顺式维甲酸。
The amounts of endogenous retinyl palmitate, retinol and retinaldehyde were measured in the neural retina and retinal pigment epithelium (RPE) of predominantly cone (chicken), rod (rat) and more mixed (cat, human) retinae. The ratio of 11-cis to all-trans isomers of retinyl palmitate and retinol in the neural retina and the RPE increases progressively with the increase in diurnality of the species from rat to chicken. The membrane fractions of both chicken and bovine RPE enzymically isomerize all-trans retinol to 11-cis-retinol. Chicken neural retina membranes enzymically form 11-cis-retinol and all-trans-retinyl palmitate from all-trans-retinol. Light and electron microscopy revealed no contamination of chicken neural retina by RPE. Muller cells from chicken retina were isolated, cultured and chracterized by immunocytochemical localization of cellular retinaldehyde-binding protein. Cultured chicken Muller cells form all-trans-retinyl palmitate, 11-cis-retinol and 11-cis-retinyl palmitate from all-trans-retinol and release most of the 11-cis-retinol into the medium. The results indicate that chicken neural retina and Muller cells in particular synthesize 11-cis-retinoids from all-trans-retinol.