DISCORDANCE OF EXOCRINE AND ENDOCRINE GROWTH AFTER 90-PERCENT PANCREATECTOMY IN RATS
DISCORDANCE OF EXOCRINE AND ENDOCRINE GROWTH AFTER 90-PERCENT PANCREATECTOMY IN RATS
复制标题
DOI：
10.2337/diabetes.37.2.232
复制
发表时间：
1988-02-01
期刊：
影响因子：
7.7
通讯作者：
BONNERWEIR, S
中科院分区：
文献类型：
--
作者：
BROCKENBROUGH, JS;WEIR, GC;BONNERWEIR, S
正如我们之前所表明的，在大鼠 90% 胰腺切除术 (PX) 后 8 周，外分泌和内分泌胰腺都有相当大的再生。在这项研究中，我们通过跟踪胰岛素、胰高血糖素和淀粉酶的胰腺含量以及外分泌和胰岛β-细胞的有丝分裂指数来检查PX后3、7、14和21天的外分泌和内分泌组织的生长。 7 天时，胰腺残余物的重量超过了假手术中解剖学上等效的组织（残余物等效物）的重量。如有丝分裂指数所示，在 PX 后的最初几周内，外分泌组织和内分泌 β-细胞的生长不一致。假手术动物的外分泌细胞和β-细胞的有丝分裂指数(以秋水仙碱处理4小时后累积的有丝分裂数来测量)较低并且在不同时间点没有变化(约0.5％)。 PX后第3天和第7天，外分泌细胞和β细胞的有丝分裂指数是假手术动物的三到四倍。在PX后14天，外分泌细胞的有丝分裂指数略有升高，尽管是显着的，而β-细胞的有丝分裂指数仍然是假手术细胞的两倍。到21天时，外分泌组织的有丝分裂指数没有差异，但PX动物上的β-细胞的有丝分裂指数仍然是假手术组的两倍。另一个生长指标，即细胞出生率，是根据秋水仙碱后 1、2、3 和 4 小时累积的有丝分裂频率回归线的斜率在 7 天时估计的。 胰腺残余物中外分泌细胞（PX 3.8.+-.0.96%，假手术0.96.+-.0.72%）和β-细胞（PX 7.2.+-.1.7%，假手术0.72.+-.0.96%）的细胞出生率均显着升高。因此，外分泌细胞和内分泌β-细胞在PX后的最初3周内经历相当大的生长。然而，它们之间的成长却存在着不一致。此外，这些成体β细胞的生长速度比以前认为的更快、更持久。因此，PX 模型可以成为研究外分泌和内分泌再生机制的有用模型。
As we have previously shown, by 8 wk after 90% pancreatectomy (PX) in the rat, there is considerable regeneration of both exocrine and endocrine pancreas. In this study we examine the growth of both the exocrine and endocrine tissue 3, 7, 14, and 21 days after PX by following the pancreatic content of insulin, glucagon, and amylase as well as the mitotic indices for exocrine and islet .beta.-cells. By 7 days the pancreatic remnant weighed more than the anatomically equivalent tissue in the sham, the remnant equivalent. The growth of the exocrine tissue and the endocrine .beta.-cells was discordant during these initial weeks after PX, as shown by the mitotic index. The mitotic index, measured as accumulated mitotic figures after a 4-h colchicine treatment, for both the exocrine and .beta.-cells in the sham animals was low and unchanging at the different time points (.apprx. 0.5%). At 3 and 7 days after PX, both the exocrine and .beta.-cells had mitotic indices three- to fourfold that of the sham animals. At 14 days after PX, the exocrine cells had a slightly, albeit significantly, elevated mitotic index, whereas that of the .beta.-cells was still double that of the shams. By 21 days there was no difference in mitotic index for exocrine tissue, but the .beta.-cells on the PX animals had a mitotic index still double that of the shams. Another index of growth, the cell birthrate, was estimated at 7 days from the slope of regression lines of the mitotic frequency accumulated 1, 2, 3, and 4 h after colchicine. The cell birthrates of both the exocrine cells (PX 3.8 .+-. 0.96%, sham 0.96 .+-. 0.72%) and the .beta.-cells (PX 7.2 .+-. 1.7%, sham 0.72 .+-. 0.96%) were significantly elevated in the pancreatic remnant. Thus, both exocrine cells and endocrine .beta.-cells undergo considerable growth in the initial 3 wk after PX. However, there is discordance in their growth with respect to each other. In addition, these adult .beta.-cells have a greater and more sustained growth than previously thought possible. The PX model could therefore become a useful model for studying the mechanisms of both exocrine and endocrine regeneration.