Secure splenic delivery of plasmid DNA and its application to DNA vaccine.
Secure splenic delivery of plasmid DNA and its application to DNA vaccine.
复制标题
质粒 DNA 的安全脾递送及其在 DNA 疫苗中的应用。
DOI：
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发表时间：
2013
影响因子：
2
通讯作者：
H. Sasaki
中科院分区：
文献类型：
--
作者：
Tomoaki Kurosaki;Yukinobu Kodama;Takahiro Muro;N. Higuchi;Tadahiro Nakamura;T. Kitahara;M. Miyakoda;K. Yui;H. Sasaki
In this experiment, we developed a novel safe and effective gene delivery vector coated with γ-polyglutamic acid (γ-PGA-coated complexes). The γ-PGA-coated complex was composed of chiseled spherical nano-particles with anionic charges. The plasmid DNA/polyethyleneimine complex (non-coated complex) showed high transgene efficiency in the spleen and lung after intravenous administration in mice, with high liver toxicity and lethality. On the other hand, γ-PGA-coated complex selectively showed high transgene efficiency in the spleen without such toxicity. Furthermore, the γ-PGA-coated complex highly accumulated and showed high gene expression in the marginal zone of the spleen. Those results strongly indicated that γ-PGA-coated complex was suitable as a DNA vaccine vector. We therefore applied γ-PGA-coated complex to melanoma DNA vaccine, pUb-M. The γ-PGA-coated complex containing pUb-M significantly inhibited the growth and metastasis of a melanoma cell line, B16-F10 cells. In conclusion, we developed a splenic gene vector, γ-PGA-coated complex, as a novel technology for clinical vaccination.
DOI：
10.1073/pnas.090097697
发表时间：
2000-05-09
影响因子：
11.1
作者：
Xiang, R;Lode, HN;Reisfeld, RA
通讯作者：
Reisfeld, RA