Vibrio caribbeanicus sp nov., isolated from the marine sponge Scleritoderma cyanea
Vibrio caribbeanicus sp nov., isolated from the marine sponge Scleritoderma cyanea
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DOI：
10.1099/ijs.0.032375-0
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发表时间：
2012-08-01
影响因子：
2.8
通讯作者：
Brown, Eric W.
中科院分区：
文献类型：
--
作者：
Hoffmann, Maria;Monday, Steven R.;Brown, Eric W.
从库拉索岛西海岸795英尺(242米)深的海绵中分离到一株革兰氏阴性、氧化酶阳性、过氧化氢酶阴性、兼性厌氧、游动、弯曲的杆状细菌N384(T)。根据16S rRNA基因序列分析，菌株N384(T)属于弧菌属，与巴西弧菌LMG 20546(T)(98.8%)、黑色脉动弧菌ATCC 27043(T)(98.5%)、途氏弧菌ATCC 19109(T)(98.6%)和中华弧菌DSM 21326(T)(98.2%)关系最近。菌株N384(T)的DNA G+C含量为41.6mol%。对5个基因(gyrB、rpoA、pyrH、mreB和FtsZ；4068bp)的串联序列分析表明，菌株N384(T)与其最邻近的菌株N384(T)之间存在明显的分离，并将菌株N384(T)聚为弧菌的东方分支。表型上，新种属于精氨酸二水解酶阳性、赖氨酸脱羧酶和鸟氨酸脱羧酶阴性(A+/L-/O-)簇。新种还根据脂肪酸组成进行了区分，特别是iso-C-13：0、iso-C-15：0、C-15：0、iso-C-16：0、C-16：0、iso-C-17：0、C-17：1 omega 8c和C-17：0的比例与巴西弧菌和中华弧菌有显著差异。DNA-DNA杂交、平均核苷酸同源性和生理生化试验的结果进一步将该菌株与其他已描述的弧菌属区分开来。总之，这些发现证实了菌株N384(T)代表了一个新的弧菌物种，其名称为caribbeanicus sp。11月模式菌株N384(T)(=ATCC BAA-2122(T)=DSM 23640(T))。
A Gram-negative, oxidase-positive, catalase-negative, facultatively anaerobic, motile, curved rod-shaped bacterium, strain N384(T), was isolated from a marine sponge (Scleritoderma cyanea; phylum Porifera) collected from a depth of 795 feet (242 m) off the west coast of Curacao. On the basis of 16S rRNA gene sequencing, strain N384(T) was shown to belong to the genus Vibrio, most closely related to Vibrio brasiliensis LMG 20546(T) (98.8 % similarity), Vibrio nigripulchritudo ATCC 27043(T) (98.5%), Vibrio tubiashii ATCC 19109(T) (98.6%) and V. sinaloensis DSM 21326(T) (98.2 %). The DNA G + C content of strain N384(T) was 41.6 mol%. An analysis of concatenated sequences of five genes (gyrB, rpoA, pyrH, mreB and ftsZ; 4068 bp) demonstrated a clear separation between strain N384(T) and its closest neighbours and clustered strain N384(T) into the 'Orientalis' clade of vibrios. Phenotypically, the novel species belonged to the arginine dihydrolase-positive, lysine decarboxylase- and ornithine decarboxylase-negative (A+/L-/O-) cluster. The novel species was also differentiated on the basis of fatty acid composition, specifically that the proportions of iso-C-13:0, iso-C-15:0, C-15:0, iso-C-16:0, C-16:0, iso-C-17:0, C-17:1 omega 8c and C-17:0 were significantly different from those found in V. brasiliensis and V. sinaloensis. The results of DNA-DNA hybridization, average nucleotide identity and physiological and biochemical tests further allowed differentiation of this strain from other described species of the genus Vibrio. Collectively, these findings confirm that strain N384(T) represents a novel Vibrio species, for which the name Vibrio caribbeanicus sp. nov. is proposed, with the type strain N384(T) (=ATCC BAA-2122(T)=DSM 23640(T)).