Integrated transcriptomics and metabolomics analysis reveals key regulatory network that response to cold stress in common Bean (Phaseolus vulgaris L.).
Integrated transcriptomics and metabolomics analysis reveals key regulatory network that response to cold stress in common Bean (Phaseolus vulgaris L.).
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DOI：
10.1186/s12870-023-04094-1
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发表时间：
2023-02-09
影响因子：
5.3
通讯作者：
Liu, Dajun
中科院分区：
文献类型：
--
作者：
Yang, Xiaoxu;Liu, Chang;Li, Mengdi;Li, Yanmei;Yan, Zhishan;Feng, Guojun;Liu, Dajun
寒冷的气温可能对作物的生存和生产力有害。通过了解耐低温的分子基础，可以提高育种进展。本研究以普通菜豆品种120和093为材料，分别研究了与抗寒性相关的关键代谢途径和关键代谢产物。通过转录组学和代谢组学的研究，发现了许多与低温胁迫反应过程中主要代谢途径有关的潜在基因和代谢产物。低温胁迫下，两种菜豆中许多与脂类、氨基酸和类黄酮代谢有关的基因表达量增加，代谢产物积累增加。丙二醛（MDA）含量在120低于093。关于氨基酸代谢，120具有比093更高的酸性氨基酸浓度，而093具有更高的碱性氨基酸浓度。120中的甲硫氨酸积累明显高于093。此外，120比093具有更高浓度的许多类型的黄酮类化合物。黄酮、蛋氨酸和丙二醛可作为植物冷害的生物标志物。对激素代谢的转录组分析表明，低温胁迫下093的阿坝、GA和JA的表达量明显高于120，表明093和120的激素调控模式不同。因此，093和120之间的耐冷性不同，可能是由于转录和代谢调控。
Cold temperatures can be detrimental to crop survival and productivity. Breeding progress can be improved by understanding the molecular basis of low temperature tolerance. We investigated the key routes and critical metabolites related to low temperature resistance in cold-tolerant and -sensitive common bean cultivars 120 and 093, respectively. Many potential genes and metabolites implicated in major metabolic pathways during the chilling stress response were identified through transcriptomics and metabolomics research. Under chilling stress, the expression of many genes involved in lipid, amino acid, and flavonoid metabolism, as well as metabolite accumulation increased in the two bean types. Malondialdehyde (MDA) content was lower in 120 than in 093. Regarding amino acid metabolism, 120 had a higher concentration of acidic amino acids than 093, whereas 093 had a higher concentration of basic amino acids. Methionine accumulation was clearly higher in 120 than in 093. In addition, 120 had a higher concentration of many types of flavonoids than 093. Flavonoids, methionine and malondialdehyde could be used as biomarkers of plant chilling injury. Transcriptome analysis of hormone metabolism revealed considerably greater, expression of abscisic acid (ABA), gibberellin (GA), and jasmonic acid (JA) in 093 than in 120 during chilling stress, indicating that hormone regulation modes in 093 and 120 were different. Thus, chilling stress tolerance is different between 093 and 120 possibly due to transcriptional and metabolic regulation.
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DOI：
10.1016/s1389-0344(02)00003-5
发表时间：
2002-06-01
期刊：
BIOMOLECULAR ENGINEERING
影响因子：
--
作者：
Buchholz, A;Hurlebaus, J;Takors, R
通讯作者：
Takors, R
影响因子：
5.6
作者：
Anwar A;Bai L;Miao L;Liu Y;Li S;Yu X;Li Y
通讯作者：
Li Y
影响因子：
0.7
作者：
Battal, Peyami;Erez, Mehmet E.;Berber, Ismet
通讯作者：
Berber, Ismet
影响因子：
3.5
作者：
Guan, Shixin;Xu, Quan;Guo, Zhifu
通讯作者：
Guo, Zhifu
影响因子：
--
作者：
Bulgari, Roberta;Turconi, Paolo;Ferrante, Antonio
通讯作者：
Ferrante, Antonio