AMPA receptor subunits define properties of state-dependent synaptic plasticity
AMPA receptor subunits define properties of state-dependent synaptic plasticity
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DOI：
10.1113/jphysiol.2010.187229
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发表时间：
2010-06-01
影响因子：
5.5
通讯作者：
Madison, Daniel V.
中科院分区：
文献类型：
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作者：
Emond, Michelle R.;Montgomery, Johanna M.;Madison, Daniel V.
许多突触通过属于突触可塑性保护伞下的过程经历强度的立即和持久的活动依赖性变化。众所周知，这种类型的突触可塑性表现出潜在的状态依赖性;也就是说，当突触强度变化时，它们进入由机制和经历未来可塑性的能力定义的不同“状态”。在这项研究中，我们已经调查的分子机制，根据状态依赖性突触可塑性。使用模拟GluR 1和GluR 2 AMPA受体亚型的C-末端尾部序列的肽的细胞内应用，结合成对的最小突触连接的记录，我们已经表明，AMPA受体亚型存在于膜中在给定的时间赋予可塑性状态的一些特性。这些数据表明，在突触可塑性，AMPA受体亚型的差异稳定的突触后膜内或外的突触后密度蛋白，这种差异的突触表达不同的AMPA受体亚型定义不同的突触状态。
Many synapses undergo immediate and persistent activity-dependent changes in strength via processes that fall under the umbrella of synaptic plasticity. It is known that this type of synaptic plasticity exhibits an underlying state dependence; that is, as synapses change in strength they move into distinct 'states' that are defined by the mechanism and ability to undergo future plasticity. In this study, we have investigated the molecular mechanisms that underlie state-dependent synaptic plasticity. Using intracellular application of peptides that mimic the C-terminal tail sequences of GluR1 and GluR2 AMPA receptor subtypes, combined with paired recordings of minimal synaptic connections, we have shown that AMPA receptor subtypes present in the membrane at a given time confer some properties of plasticity states. These data show that during synaptic plasticity, AMPA receptor subtypes are differentially stabilized by postsynaptic density proteins in or out of the postsynaptic membrane, and this differential synaptic expression of different AMPA receptor subtypes defines distinct synaptic states.