Transport of a cell-sized phospholipid micro-container across water/oil interface
Transport of a cell-sized phospholipid micro-container across water/oil interface
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DOI：
10.1016/j.cplett.2006.06.016
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发表时间：
2006-08-04
影响因子：
2.8
通讯作者：
Yoshikawa, Kenichi
中科院分区：
文献类型：
--
作者：
Hase, Masahiko;Yamada, Ayako;Yoshikawa, Kenichi
当将直径为几十μ m的细胞大小的水滴置于含有磷脂的油中时，在液滴表面上自发形成磷脂单层，以产生稳定的细胞大小的微容器。我们报告这样的“囊泡”之间的油相和水相的运输在散装水/油界面的脂质单层显微操作技术。“囊泡”可以返回到油相上，并且可以在两相之间反复进行来回输送。这种囊泡状的、易于操作的微容器可以提供活的细胞内环境的简单模型。(c)2006 Elsevier B. V.保留所有权利。
When a cell-sized water droplet, with a diameter of several tens of mu m, is placed in oil containing phospholipids, a phospholipid monolayer is formed spontaneously on the droplet surface to produce a stable cell-sized micro-container. We report on the transportation of such 'vesicle' between an oil and a water phases across a lipid monolayer at the bulk water/oil interface by micromanipulation technique. The 'vesicle' can be returned onto the oil phase, and the transportation can be performed back and forth between the two-phases repeatedly. Such a vesicle-like, easily manipulable micro-container may afford a simple model of living intra-cellular environments. (c) 2006 Elsevier B.V. All rights reserved.