Effective Treatment of Bladder Tumor-Bearing Mice by Direct Delivery of Bleomycin Using Electrochemotherapy
Effective Treatment of Bladder Tumor-Bearing Mice by Direct Delivery of Bleomycin Using Electrochemotherapy
复制标题
电化学疗法直接递送博莱霉素有效治疗膀胱肿瘤小鼠
DOI：
10.1080/10717540260397864
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发表时间：
2002
期刊：
影响因子：
6
通讯作者：
K. Yoshikawa
中科院分区：
文献类型：
--
作者：
K. Mitsui;Keitaro Kato;Kogenta Nakamura;Y. Yamada;N. Honda;H. Fukatsu;K. Yoshikawa
我们研究了博来霉素 (BLM) 与电脉冲组合对裸鼠移植膀胱肿瘤的细胞毒性。注射 BLM 后施加高压电脉冲与肿瘤尺寸减小相关；治疗后8天，肿瘤尺寸变小并消失。这种完全消退是通过使用致死剂量十分之一 (LD 50) 的 BLM 结合电脉冲进行的一种治疗实现的。第 20 天，观察到再生速度比较低浓度 BLM 慢得多。治疗后肿瘤组织的病理检查显示，第2天暴露组织中的细胞核破裂。此外，第4天观察到染色细胞核数量减少和细胞质溶解。这些结果表明，电脉冲是将药物递送到膀胱肿瘤细胞的有效工具，也是膀胱肿瘤的有效治疗方法。
We investigated the cytotoxicity on the combination of bleomycin (BLM) with electric pulses against transplanted bladder tumors in nude mice. Loading with high-voltage electric pulse after injection of BLM was associated with a decrease in tumor size; tumor size became smaller and disappeared 8 days after the treatment. Such complete regression was achieved with one treatment using BLM of one-tenth the lethal dose (LD 50) combined with electric pulses. On day 20, regrowth was observed at a much slower rate than at lower concentrations of BLM. Pathological examinations of the tumor tissues after treatment revealed disruption of the nucleus in the exposed tissues on day 2. Furthermore, a decrease in number of the stained nuclei and cytoplasmic lysis were observed on day 4. These results showed that electric pulses was an effective tool to deliver drugs into bladder tumor cells and an effective treatment method for bladder tumors.