Anti-angiogenic and anti-inflammatory effect of Magnolol in the oxygen-induced retinopathy model
Anti-angiogenic and anti-inflammatory effect of Magnolol in the oxygen-induced retinopathy model
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厚朴酚在氧诱导视网膜病变模型中的抗血管生成和抗炎作用
DOI：
10.1007/s00011-015-0894-x
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发表时间：
2015
影响因子：
6.7
通讯作者：
Liang, Xiaoling
中科院分区：
文献类型：
--
作者：
Yu, Shanshan;Huang, Yongsheng;Lu, Lin;Liang, Xiaoling
本研究采用氧诱导视网膜病变（OIR）模型，观察厚朴酚（Magnolol）对视网膜新生血管（RNV）和局部神经胶质细胞的影响，并探讨其分子机制。从P12至P17每天一次腹腔注射25 mg/kg厚朴酚，然后收获视网膜并平放以评估视网膜血管、星形胶质细胞和小胶质细胞。为了阐明厚朴酚的分子机制，我们观察了炎症细胞因子如白细胞介素（IL）-1β、IL-6、单核细胞趋化蛋白-1、肿瘤坏死因子-α、分析缺氧诱导因子（HIF）-1α/血管内皮生长因子（VEGF）结果腹腔注射厚朴酚可显著降低RNV，而无视网膜毒性或干扰RNV。发育性视网膜血管生成此外，厚朴酚保留了星形胶质细胞的形态，并减少了小胶质细胞的活化。结论厚朴酚可能通过抗炎和抑制HIF-1 α/VEGF通路，对视网膜病理性血管生成和胶质细胞功能障碍具有治疗作用。
ObjectiveIn the present study, we investigated the effects of Magnolol on the retinal neovascularization (RNV) and local glial cells in an oxygen-induced retinopathy (OIR) model and explored their molecular mechanisms.Materials and methodsNeonatal C57BL/6J mice were subjected to 75 % O2± 5 % from postnatal day (P) 7 to P12 and subsequently returned to room air. Mice were injected with 25 mg/kg Magnolol intraperitoneally once a day from P12 to P17, then retinas were harvested and flat-mounted to assess the retinal vessels, astrocytes and microglia. To clarify the molecular mechanisms of Magnolol, we observed the level of inflammatory cytokines such as interleukin (IL)-1β, IL-6, monocyte chemoattractant protein-1, tumor necrosis factor-α, and analyzed the hypoxia-inducible factor (HIF)-1α/vascular endothelial growth factor (VEGF) pathway in OIR mice.ResultsIntraperitoneal administration of Magnolol resulted in significant reduction of RNV without retinal toxicity or perturbation of developmental retinal angiogenesis. In addition, Magnolol preserved the astrocyte morphology and diminished the activation of microglia. Moreover, Magnolol down regulated the expression of inflammatory cytokines and inactivated the HIF-1α/VEGF pathway.ConclusionsThese results indicated that Magnolol might have potential for the treatment of pathological retinal angiogenesis and glial dysfunctions via anti-inflammation and inhibition of HIF-1α/VEGF pathway.
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影响因子：
9.3
作者：
Chuang DY;Chan MH;Zong Y;Sheng W;He Y;Jiang JH;Simonyi A;Gu Z;Fritsche KL;Cui J;Lee JC;Folk WR;Lubahn DB;Sun AY;Sun GY
通讯作者：
Sun GY
影响因子：
9
作者：
Kumar S;Guru SK;Pathania AS;Kumar A;Bhushan S;Malik F
通讯作者：
Malik F
在缺血性视网膜病变模型中，视网膜血管修复和新生血管形成不依赖于 CX3CR1 信号传导。
影响因子：
3.4
作者：
Zhao, Lian;Ma, Wenxin;Fariss, Robert N.;Wong, Wai T.
通讯作者：
Wong, Wai T.
影响因子：
5.3
作者：
STONE, J;ITIN, A;KESHET, E
通讯作者：
KESHET, E
影响因子：
3.9
作者：
Falavarjani, K. Ghasemi;Nguyen, Q. D.
通讯作者：
Nguyen, Q. D.