Microfluidic cell counter/sorter utilizing multiple particle tracing technique and optically switching approach
Microfluidic cell counter/sorter utilizing multiple particle tracing technique and optically switching approach
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DOI：
10.1007/s10544-007-9109-8
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发表时间：
2008-02-01
影响因子：
2.8
通讯作者：
Lin, Che-Hsin
中科院分区：
文献类型：
--
作者：
Lin, Chen-Chen;Chen, Angela;Lin, Che-Hsin
本文提出了一种基于计算机控制的数字图像处理(DIP)技术和光钳的新型微流控系统，用于连续流动环境中细胞/微粒的自动识别、计数和分选。在所提出的系统中，细胞/微粒被电动聚焦成狭窄的样本流，然后被驱动通过感兴趣区域(ROI)，在那里使用专有的DIP系统实时识别和跟踪它们。然后，使用由DIP系统产生的同步控制信号来激励聚焦的IR激光束，以将目标细胞从主样本流移位到相邻的鞘流中，该鞘流将它们携带到下游收集通道，在那里它们被自动计数。实验表明，该芯片能够连续分选和计数直径为5和10微米的微粒，并成功地分选和收集了由酵母细胞和聚苯乙烯(PS)小球组成的样品，产率为100%，回收率为91.9%。该系统为微流控器件中的细胞操作提供了一种简单、低成本、高性能的解决方案。
This paper proposes a novel microfluidic system based on a computer controlled digital image processing (DIP) technique and optical tweezers for automatic cell/microparticle recognition, counting and sorting in a continuous flow environment. In the proposed system, the cells/microparticles are focused electrokinetically into a narrow sample stream and are then driven through the region of interest (ROI), where they are recognized and traced in real time using a proprietary DIP system. Synchronized control signals generated by the DIP system are then used to actuate a focused IR laser beam to displace the target cells from the main sample stream into a neighboring sheath flow, which carries them to a downstream collection channel where they are automatically counted. Experimental trials show that the microchip is capable of continuously sorting and counting microparticles with diameters of 5 and 10 mu m. In addition, a sample composed of yeast cells and polystyrene (PS) beads is successfully sorted and collected with a 100% of yield ratio and 91.9% of recovery ratio. The proposed system provides a simple, low-cost, high-performance solution for cell manipulation in microfluidic devices.