A microfluidic-enabled mechanical microcompressor for the immobilization of live single- and multi-cellular specimens.
A microfluidic-enabled mechanical microcompressor for the immobilization of live single- and multi-cellular specimens.
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DOI：
10.1017/s1431927613014037
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发表时间：
2014-02
期刊：
影响因子：
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通讯作者：
Janetopoulos C
中科院分区：
文献类型：
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作者：
Yan Y;Jiang L;Aufderheide KJ;Wright GA;Terekhov A;Costa L;Qin K;McCleery WT;Fellenstein JJ;Ustione A;Robertson JB;Johnson CH;Piston DW;Hutson MS;Wikswo JP;Hofmeister W;Janetopoulos C
微型压缩机是一种精密的机械设备，它可以将活细胞和小生物体固定在光学显微镜下，从而增强亚细胞结构和细胞器的可视化。我们已经开发出一种易于制造的设备，它可以配备微流体，允许在观察过程中添加介质或化学品。该装置可用于直立和倒置显微镜。该装置允许微米精度平整的无损固定标本小如细菌，同时也容纳较大的标本，如秀丽隐杆线虫，长期观察。压缩机安装是可拆卸的，并允许方便的样品添加和回收，以便以后观察。几个定制的标本床可以纳入基地。为了证明该设备的能力，我们已经在几种原生动物物种，酵母细胞和果蝇胚胎中成像了许多细胞事件。我们已经能够记录以前未报道的事件，也进行光漂白实验，在共轭四膜虫嗜热。
A microcompressor is a precision mechanical device that flattens and immobilizes living cells and small organisms for optical microscopy, allowing enhanced visualization of sub-cellular structures and organelles. We have developed an easily fabricated device, which can be equipped with microfluidics, permitting the addition of media or chemicals during observation. This device can be used on both upright and inverted microscopes. The apparatus permits micrometer precision flattening for nondestructive immobilization of specimens as small as a bacterium, while also accommodating larger specimens, such as Caenorhabditis elegans, for long-term observations. The compressor mount is removable and allows easy specimen addition and recovery for later observation. Several customized specimen beds can be incorporated into the base. To demonstrate the capabilities of the device, we have imaged numerous cellular events in several protozoan species, in yeast cells, and in Drosophila melanogaster embryos. We have been able to document previously unreported events, and also perform photobleaching experiments, in conjugating Tetrahymena thermophila.