Genome-wide identification and characterisation of Aquaporins inNicotiana tabacumand their relationships with other Solanaceae species
Genome-wide identification and characterisation of Aquaporins inNicotiana tabacumand their relationships with other Solanaceae species
复制标题
DOI：
10.1186/s12870-020-02412-5
复制
发表时间：
2020-06-09
影响因子：
5.3
通讯作者：
Groszmann, Michael
中科院分区：
文献类型：
--
作者：
De Rosa, Annamaria;Watson-Lazowski, Alexander;Groszmann, Michael
细胞膜是动态结构，不断调整其组成，使植物能够响应发育信号、胁迫和变化的环境。为了促进底物的跨膜运输，植物膜上同时嵌入了主动和被动转运蛋白。水通道蛋白（AQPs）是一个主要的跨膜通道蛋白家族，可选择性地促进底物以每秒10(8)个分子的速度被动双向通过生物膜。AQPs在植物界最为多样化，包括5个主要亚家族，它们在时间和空间基因表达、亚细胞蛋白定位、底物特异性和翻译后调控机制方面存在差异；共同提供一个跨越整个工厂的动态运输网络。植物AQPs可以运输一系列溶质，这些溶质对许多植物过程至关重要，包括水分关系、生长发育、胁迫响应、根系养分吸收和光合作用。操纵aqp以提高植物生产力的能力依赖于我们对aqp多样性和功能作用的深入了解。结果：我们对烟草中的AQP家族进行了表征（NtAQPs；烟草），这是一种能够从实验室扩展到现场的流行模型系统。烟草与主要经济作物（如番茄、马铃薯、茄子和辣椒）密切相关，本身具有新的商业用途。烟草含有76个AQP，使其成为第二大AQP家族。它们分为五个不同的亚家族，我们描述了它们的系统发育关系、基因结构、蛋白质序列、选择性过滤器组成、亚细胞定位和组织特异性表达。我们还从烟草亲本基因组(N. sylvestrisn .和N.；这使我们能够描述NtAQP家族的进化史。对番茄和马铃薯AQPs进行同源性鉴定，可以跨物种比较它们在蛋白质结构、基因表达和潜在生理作用方面的保守性。结论本研究提供了烟草AQP家族的全面特征，加强了AQP生物学的现有知识。精细化的基因/蛋白质模型、组织特异性表达分析和跨物种比较，为NtAQPs及其茄科同源植物的进化历史和可能的生理作用提供了有价值的见解。总的来说，这些结果将支持未来的功能研究，并有助于将基础研究转移到应用农业。
Background Cellular membranes are dynamic structures, continuously adjusting their composition, allowing plants to respond to developmental signals, stresses, and changing environments. To facilitate transmembrane transport of substrates, plant membranes are embedded with both active and passive transporters. Aquaporins (AQPs) constitute a major family of membrane spanning channel proteins that selectively facilitate the passive bidirectional passage of substrates across biological membranes at an astonishing 10(8)molecules per second. AQPs are the most diversified in the plant kingdom, comprising of five major subfamilies that differ in temporal and spatial gene expression, subcellular protein localisation, substrate specificity, and post-translational regulatory mechanisms; collectively providing a dynamic transportation network spanning the entire plant. Plant AQPs can transport a range of solutes essential for numerous plant processes including, water relations, growth and development, stress responses, root nutrient uptake, and photosynthesis. The ability to manipulate AQPs towards improving plant productivity, is reliant on expanding our insight into the diversity and functional roles of AQPs. Results We characterised the AQP family fromNicotiana tabacum(NtAQPs; tobacco), a popular model system capable of scaling from the laboratory to the field. Tobacco is closely related to major economic crops (e.g. tomato, potato, eggplant and peppers) and itself has new commercial applications. Tobacco harbours 76 AQPs making it the second largest characterised AQP family. These fall into five distinct subfamilies, for which we characterised phylogenetic relationships, gene structures, protein sequences, selectivity filter compositions, sub-cellular localisation, and tissue-specific expression. We also identified the AQPs from tobacco's parental genomes (N. sylvestrisandN. tomentosiformis), allowing us to characterise the evolutionary history of the NtAQP family. Assigning orthology to tomato and potato AQPs allowed for cross-species comparisons of conservation in protein structures, gene expression, and potential physiological roles. Conclusions This study provides a comprehensive characterisation of the tobacco AQP family, and strengthens the current knowledge of AQP biology. The refined gene/protein models, tissue-specific expression analysis, and cross-species comparisons, provide valuable insight into the evolutionary history and likely physiological roles of NtAQPs and their Solanaceae orthologs. Collectively, these results will support future functional studies and help transfer basic research to applied agriculture.