Understanding the Limitations in the Biosynthesis of Polyhydroxyalkanoate (PHA) from Lignin Derivatives
Understanding the Limitations in the Biosynthesis of Polyhydroxyalkanoate (PHA) from Lignin Derivatives
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DOI：
10.1021/sc500066f
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发表时间：
2014-05-01
影响因子：
8.4
通讯作者：
Numata, Keiji
中科院分区：
文献类型：
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作者：
Tomizawa, Satoshi;Chuah, Jo-Ann;Numata, Keiji
为了研究木质素衍生物的生物聚酯的转化，11聚羟基链烷酸酯（PHA）积累菌株培养的矿物盐培养基中含有的18个木质素衍生物和羟基苯甲酸，包括中间体衍生的木质素衍生物在细菌中的代谢。大多数菌株在含有木质素衍生物如对香豆酸、咖啡酸、阿魏酸和芥子酸的培养基中生长不良。其中一株恶臭假单胞菌Gpo 1在对香豆酸、阿魏酸、香草酸、4-羟基苯甲酸（4-HBA）和3，4-二羟基苯甲酸（3，4-DHBA）存在下生长。恶臭假单胞菌JCM 13063也在4-HBA、3，4-DHBA和香草酸存在下生长。另一菌株Ralstonia eutropha H16从4-HBA、2，5-DHBA和3，4-DHBA合成PHA。从这些实验中获得的数据的基础上，我们认为，木质素衍生物转化为中间体，如4-HBA，2，5-DHBA，3，4-DHBA，香草酸的主要瓶颈在合成PHA的木质素衍生物。
To investigate the conversion of lignin derivatives to biopolyesters, 11 polyhydroxyalkanoate (PHA)-accumulating strains were cultured on mineral salt media containing each of the 18 lignin derivatives and hydroxybenzoic acids, including intermediates derived from the metabolism of lignin derivatives in bacteria. Most of the strains grew poorly in media containing lignin derivatives such as p-coumaric acid, caffeic acid, ferulic acid, and sinapinic acid. One of the strains, Pseudomonas putida Gpo1, grew in the presence of p-coumaric acid, ferulic acid, vanillic acid, 4-hydroxybenzoic acid (4-HBA), and 3,4-dihydroxybenzoic acid (3,4-DHBA). Pseudomonas putida JCM 13063 also grew in the presence of 4-HBA, 3,4-DHBA, and vanillic acid. Another strain, Ralstonia eutropha H16, synthesized PHA from 4-HBA, 2,5-DHBA, and 3,4-DHBA. On the basis of the data obtained from these experiments, we suggest that the conversion of lignin derivatives into intermediates such as 4-HBA, 2,5-DHBA, 3,4-DHBA, and vanillic acid represents the major bottleneck in the synthesis of PHA from lignin derivatives.