Preparation of 3′-Phosphoadenosine 5′-Phospho35S-sulfate Using ATP Sulfurylase and APS Kinase from Bacillus stearothermophilus: Enzymatic Synthesis and Purification
Preparation of 3′-Phosphoadenosine 5′-Phospho35S-sulfate Using ATP Sulfurylase and APS Kinase from Bacillus stearothermophilus: Enzymatic Synthesis and Purification
复制标题
使用嗜热脂肪芽孢杆菌 ATP 硫酸化酶和 APS 激酶制备 3-磷酸腺苷 5-Phospho35S-硫酸盐：酶法合成和纯化
DOI：
--
复制
发表时间：
1993
期刊：
影响因子：
--
通讯作者：
M. Suiko
中科院分区：
文献类型：
--
作者：
P. H. Fernando;Atsushi Karakawa;Y. Sakakibara;H. Ibuki;H. Nakajima;Ming;M. Suiko
使用从嗜热脂肪芽孢杆菌纯化的酶合成具有预定比活性的放射性PAPS。反应体系中加入1.5mCi的35 S-H_2SO_4和500 nmol的ATP，产生582 μCi的35 S-PAPS。合成的35 S-PAPS通过ECTEOLA纤维素色谱法纯化，然后用DOWEX 50 W-X8洗脱。最终产量为206 μmol 35 S-PAPS，放射性为411 μCi。
Radioactive PAPS was synthesized with a predetermined specific activity using enzymes purified from Bacillus stearothermophilus. The reaction system, incorporating 1.5mCi of 35S-H2SO4 and 500 nmol of ATP, produced 582 μCi of 35S-PAPS. The synthesized 35S-PAPS was purified by ECTEOLA cellulose chromatography, followed by desalting with DOWEX 50W-X8. The final yield was 206 μmol of 35S-PAPS with a radioactivity of 411 μCi.