Curcumin delivery by methoxy polyethylene glycol-poly(caprolactone) nanoparticles inhibits the growth of C6 glioma cells.
Curcumin delivery by methoxy polyethylene glycol-poly(caprolactone) nanoparticles inhibits the growth of C6 glioma cells.
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DOI：
10.1093/abbs/gmr011
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发表时间：
2011-04
影响因子：
3.7
通讯作者：
J. Shao;Donghui Zheng;Zhifeng Jiang;Huae Xu;Yong Hu;Xiaolin Li;Xiaowei Lu
中科院分区：
文献类型：
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作者：
J. Shao;Donghui Zheng;Zhifeng Jiang;Huae Xu;Yong Hu;Xiaolin Li;Xiaowei Lu
姜黄素（Cum）作为一种潜在的抗癌药物，已被报道通过影响细胞周期阻滞、分化、细胞凋亡等对一系列癌症具有化学预防和化疗活性。因此，Cum对多种癌症的潜在活性增加了其作为新型模型药物应用于纳米颗粒递送系统的可能性。本文报道了一种由两亲性甲氧基聚乙二醇-聚己内酯（mPEG-PCL）嵌段共聚物形成的球形核壳结构姜黄素纳米粒（Cum-np）。表征测试表明，由于其亲脂性，Cum以高包封率被掺入mPEG-PCL基纳米颗粒中。掺入的Cum可以以持续的方式从Cum-np中释放出来。纳米颗粒通过内吞作用将精液有效地转运到细胞内，并定位在细胞核周围。体外实验证明Cum-np对大鼠C6胶质瘤细胞株具有促凋亡作用，且呈剂量依赖性。目前的结果表明，Cum-np可能是一个潜在的有用的化疗制剂恶性胶质瘤的治疗。此外，中药纳米药物制剂的发展需要对其临床应用进行更深入的研究。
As a potential anticancer agent, curcumin (Cum) has been reported for its chemopreventive and chemotherapeutic activity in a series of cancers through influencing cell cycle arrest, differentiation, apoptosis, etc. Therefore, the potential activity against various cancers of Cum raises the possibility of its application as a novel model drug in nanoparticle-based delivery systems. The current study reported a spherical core-shell structure curcumin-loaded nanoparticle (Cum-np) formed by amphilic methoxy polyethylene glycol-poly(caprolactone) (mPEG-PCL) block copolymers. Characterization tests indicated that Cum was incorporated into mPEG-PCL-based nanoparticles with high encapsulation efficiency due to its lipophilicity. The incorporated Cum could be released from Cum-np in a sustained manner. Cum was effectively transported into the cells by nanoparticles through endocytosis and localized around the nuclei in the cytoplasms. In vitro studies proved that the cytotoxicity of Cum-np would be pro-apoptosis effect against rat C6 glioma cell line in a dose-dependent manner. The present results suggest that Cum-np could be a potential useful chemotherapeutic formulation for malignant glioma therapy. Moreover, the development of traditional Chinese medicine with nanoscale drug formation warrants more intensive research for its clinical applications.