Plant identity exerts stronger effect thanfertilization on soil arbuscular mycorrhizal fungi in a sown pasture
Plant identity exerts stronger effect thanfertilization on soil arbuscular mycorrhizal fungi in a sown pasture
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植物特性对播种牧场土壤丛枝菌根真菌的影响比施肥更强
DOI：
10.1007/s00248-016-0817-6
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发表时间：
2016
影响因子：
3.6
通讯作者：
Guo Liang-Dong
中科院分区：
文献类型：
--
作者：
Zheng Yong;Chen Liang;Luo Cai-Yun;Zhang Zhen-Hua;Wang Shi-Ping;Guo Liang-Dong
丛枝菌根真菌在植物营养和植物生产中起着重要作用。AM真菌对植物身份或施肥的反应已经被研究。然而，不同植物种类和肥料类型对这些共生真菌的相互作用仍然知之甚少。我们评估了植物特性（grassesAvena sativa和Escherichus nutans和豆科植物豌豆）和施肥（尿素和羊粪）的因子组合对AM真菌的影响，在人工草地研究中，在2年的单作后。AM真菌根外菌丝密度显著高于E. nutans比that in A. sativa和V.在不施肥的对照中，尿素和有机肥显著提高了A.在E. nutans，但不是由任何肥料inV。sativa. AM真菌孢子密度没有显着影响植物身份或施肥。通过18 SrDNA的454焦磷酸测序，获得了48个AM真菌操作分类单位（OTUs）。AM真菌OTU丰富度和Shannon多样性指数均显著高于E。nutans比那些inV. sativa和/或A. sativa，但在所有三种植物中不受任何肥料的显著影响。AM真菌群落组成的显着结构直接通过植物身份，只有间接通过尿素添加和植物身份，通过土壤全氮含量。我们的研究结果表明，植物身份比施肥对地下AM真菌群落的影响更大，在这个转换牧场从高寒草甸。
Arbuscular mycorrhizal (AM) fungi play key roles in plant nutrition and plant productivity. AM fungal responses to either plant identity or fertilization have been investigated. However, the interactive effects of different plant species and fertilizer types on these symbiotic fungi remain poorly understood. We evaluated the effects of the factorial combinations of plant identity (grassesAvena sativaandElymus nutansand legumeVicia sativa) and fertilization (urea and sheep manure) on AM fungi following 2-year monocultures in a sown pasture field study. AM fungal extraradical hyphal density was significantly higher inE. nutansthan that inA. sativaandV. sativain the unfertilized control and was significantly increased by urea and manure inA. sativaand by manure only inE. nutans, but not by either fertilizers inV. sativa. AM fungal spore density was not significantly affected by plant identity or fertilization. Forty-eight operational taxonomic units (OTUs) of AM fungi were obtained through 454 pyrosequencing of 18S rDNA. The OTU richness and Shannon diversity index of AM fungi were significantly higher inE. nutansthan those inV. sativaand/orA. sativa, but not significantly affected by any fertilizer in all of the three plant species. AM fungal community composition was significantly structured directly by plant identity only and indirectly by both urea addition and plant identity through soil total nitrogen content. Our findings highlight that plant identity has stronger influence than fertilization on belowground AM fungal community in this converted pastureland from an alpine meadow.