Plasma atrial natriuretic peptide and natriuretic peptide receptor gene expression in adipose tissue of normotensive and hypertensive obese patients
Plasma atrial natriuretic peptide and natriuretic peptide receptor gene expression in adipose tissue of normotensive and hypertensive obese patients
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DOI：
10.1097/00004872-199715120-00074
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发表时间：
1997-12-01
影响因子：
4.9
通讯作者：
Rappelli, A
中科院分区：
文献类型：
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作者：
Dessì-Fulgheri, P;Sarzani, R;Rappelli, A
目的人和大鼠脂肪组织中含有很高水平的钠尿肽清除受体信使(M)RNA，禁食可抑制其基因表达。本研究检测了27例高血压肥胖者、26例正常血压肥胖者、24例非肥胖性高血压患者和23例健康体检者血浆心钠素(ANP)水平及脂肪组织中生物活性A型利钠肽受体(NPR-A)和清除钠尿肽受体(NPR-C)的基因表达。抽取血样进行心钠素、血浆肾素活性和醛固酮放射免疫测定。对13例肥胖性高血压患者和12例正常血压肥胖者的脐部皮下脂肪组织进行了针吸，并用于RNA提取。然后，用与NPR-A、NPR-C和β-肌动蛋白基因不同外显子序列互补的引物进行互补合成和半定量聚合酶链式反应(PCR)。P-32标记的聚合酶链式反应产物经电泳法分离，印迹到尼龙膜上，曝光的放射自显影胶片用密度计量法分析。结果肥胖高血压患者血浆ANP水平低于正常血压肥胖患者(37.5+/-7pg/ml比43.2+/-6pg/ml，P<0.05)，但非肥胖高血压患者血浆ANP水平高于非肥胖正常血压患者。相反，肥胖高血压患者的血浆肾素活性和醛固酮水平较高。虽然NPR-A和NPR-C的表达在两组肥胖者之间无统计学差异，但肥胖性高血压患者的NPR-A/NPR-C mRNA比率显著低于肥胖组(P<；0.03)。结论与正常肥胖者相比，肥胖高血压患者NPR-A/NPR-C比值降低可能决定了肥胖性高血压患者的生物学活性降低和/或钠尿肽清除增加，提示钠尿肽及其受体系统可能在心钠素水平较低的肥胖性高血压中起重要作用。(C)快速科学出版商ISSN 0263-6352。
Objective Human and rat adipose tissue contain very high levels of natriuretic peptides clearance receptor messenger (m)RNA, and fasting inhibits its gene expression in adipose tissue. In this study we evaluated plasma atrial natriuretic peptide (ANP) and gene expression of biologically active type A natriuretic peptide receptor (NPr-A) and clearance natriuretic peptide receptor (NPr-C) in adipose tissue of obese hypertensive and obese normotensive patients.Design and methods We studied 27 untreated obese hypertensives, 26 obese normotensives (body mass index greater than or equal to 30 kg/m(2)), 24 non-obese essential hypertensives and 23 lean healthy subjects (body mass index less than or equal to 25 kg/m(2)). Blood samples were withdrawn for ANP, plasma renin activity and aldosterone radioimmunoassays. Subcutaneous peri-umbilical adipose tissue samples were obtained, by needle aspiration, in 13 obese hypertensives and in 12 obese normotensives and used for RNA extraction. Then, complementary synthesis and semiquantitative polymerase chain reaction (PCR) with primers complementary to sequences of different exons of the genes encoding for NPr-A, NPr-C and beta-actin, were performed. P-32-labeled PCR products were separated by electrophoresis, blotted onto nylon membranes, and the exposed autoradiographic films were analysed by densitometry. NPr signals were normalized by the beta-actin expression level.Results Plasma ANP was lower in obese hypertensives than in obese normotensives (37.5 +/- 7 versus 43.2 +/- 6 pg/ml, P < 0.05), but was higher in non-obese hypertensives than in non-obese normotensives. In contrast, plasma renin activity and aldosterone were higher in the obese hypertensives. Although NPr-A and NPr-C expression were not statistically different between the two obese groups, the NPr-A : NPr-C mRNA ratios were significantly lower in obese hypertensives (P < 0.03).Conclusions Our data suggest that in obese hypertensives compared to obese normotensives, the lower NPr-A : NPr-C ratio might determine decreased biological activity and/or an increased clearance of natriuretic peptide in adipose tissue, suggesting that the natriuretic peptide and its receptor system may be important in obesity-related hypertension where ANP levels are lower. (C) Rapid Science Publishers ISSN 0263-6352.