Partial nitrification and nitrous oxide emission in an intermittently aerated sequencing batch biofilm reactor
Partial nitrification and nitrous oxide emission in an intermittently aerated sequencing batch biofilm reactor
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DOI：
10.1016/j.cej.2012.10.093
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发表时间：
2013-02
影响因子：
15.1
通讯作者：
Q. Kong;Jian Zhang;Ming-sheng Miao;Lin Tian;Ning Guo;S. Liang
中科院分区：
文献类型：
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作者：
Q. Kong;Jian Zhang;Ming-sheng Miao;Lin Tian;Ning Guo;S. Liang
本研究尝试在序批式生物膜反应器（SBBR）中启动短程硝化，产生适合厌氧氨氧化工艺的进水，并调查一氧化二氮（N2O）的排放。在间歇曝气条件下，在（35±1）°C下运行31d后，实现了亚硝化。长期监测表明，进入短程硝化系统的氮中约有（1.50±0.22，n=5）%以N2O形式排放。短程硝化过程中N_2O排放量（y）随时间（x）的变化规律可用y=0.0375x+0.4806，R_2 =0.9949的直线拟合。采用聚合酶链反应（PCR）和变性梯度凝胶电泳（DGGE）技术对该菌的分子生态学进行了研究。经过长期的短程硝化运行，酸性细菌、噬细胞菌和丝孢菌科细菌得到了富集。亚硝化螺菌是通过硝化细菌反硝化途径引起氧化亚氮排放的优势氨氧化物。
This study attempts to start up partial nitrification in a sequencing batch biofilm reactor (SBBR) producing an influent suitable for the anammox process and investigate the emission of nitrous oxide (N2O). After 31days of operation, partial nitrification was achieved at (35±1)°C under intermittently aerated. Based on long-term monitoring, about (1.50±0.22, n=5)% of the incoming nitrogen, loaded to the partial nitrification system, was emitted as N2O. The dynamic behavior of N2O emission (quantity (y) varying with time (x)) in the partial nitrification process can be fit to a line where y=0.0375x+0.4806, R2=0.9949. Furthermore, the molecular ecology using polymerase chain reaction (PCR) and denaturing gradient gel electrophoresis (DGGE) techniques was studied, Arcobacter and Nitrospira were washed out during to the controlled aeration and high temperature. Acidobacteria, Cytophagales and Hyphomicrobiaceae were enriched after long term operation of the partial nitrification. Nitrosospira was the dominant AOB causing nitrous oxide emission via a nitrifier denitrification pathway.