Performance of the GeneXpert CT/NG Assay Compared to That of the Aptima AC2 Assay for Detection of Rectal Chlamydia trachomatis and Neisseria gonorrhoeae by Use of Residual Aptima Samples
Performance of the GeneXpert CT/NG Assay Compared to That of the Aptima AC2 Assay for Detection of Rectal Chlamydia trachomatis and Neisseria gonorrhoeae by Use of Residual Aptima Samples
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DOI：
10.1128/jcm.01930-12
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发表时间：
2012-12-01
影响因子：
9.4
通讯作者：
Tong, C. Y. W.
中科院分区：
文献类型：
--
作者：
Goldenberg, S. D.;Finn, J.;Tong, C. Y. W.
目前还没有经监管部门批准的用于直肠拭子中沙眼衣原体和淋病奈瑟菌检测的商业化分子检测方法。我们比较了造父变星GeneXpert CT/NG检测与GenProbe Aptima Combo2检测，使用409份直肠拭子。以Aptima为金标准，GeneXpert检测沙眼衣原体和淋病奈索菌的灵敏度、特异性和阳性、阴性预测值分别为86%、99.2%、92.5%、98.4%和91.1%、100%、100%、98.6%。尽管在GeneXpert检测之前样品被显著稀释，但该检测结果表现良好，具有出色的特异性。
There are currently no commercially available molecular assays for the detection of Chlamydia trachomatis and Neisseria gonorrhoeae in rectal swabs with regulatory approval. We compared the Cepheid GeneXpert CT/NG assay with the GenProbe Aptima Combo2 assay, using 409 rectal swabs. Using Aptima as the gold standard, the sensitivity, specificity, and positive and negative predictive values of GeneXpert for the detection of C. trachomatis and N. gonorrhoeae were 86%, 99.2%, 92.5%, and 98.4% and 91.1%, 100%, 100%, and 98.6%, respectively. Despite significant dilution of samples prior to GeneXpert testing, the assay performed well with excellent specificity.