Preparation and properties of PLGA nanofiber membranes reinforced with cellulose nanocrystals
Preparation and properties of PLGA nanofiber membranes reinforced with cellulose nanocrystals
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DOI：
10.1016/j.colsurfb.2015.05.029
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发表时间：
2015-08-01
影响因子：
5.8
通讯作者：
Zhang, Yuanming
中科院分区：
文献类型：
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作者：
Mo, Yunfei;Guo, Rui;Zhang, Yuanming
聚乳酸-聚乙醇酸（PLGA）纳米纤维膜虽然在药物载体和组织工程领域得到了广泛的应用，但其生物相容性和力学性能仍然限制了其应用。本研究的目的是通过将纤维素纳米晶体（cnc）引入PLGA纳米纤维膜来提高它们的实用性。采用静电纺丝法制备了PLGA和PLGA/CNC复合纳米纤维膜，并通过扫描电镜（SEM）、热重分析（TGA）、差示扫描量热法（DSC）和动态力学分析（DMA）对其形貌和热力学力学性能进行了表征。通过WST-1实验、扫描电镜（SEM）和共聚焦激光扫描显微镜（CLSM）研究了纳米纤维膜的细胞相容性和细胞反应。加入CNC（1、3、5、7 wt.%）使制备的纳米纤维膜的平均纤维直径从100 nm（纯PLGA）增加到接近400 nm (PLGA/7 wt.% CNC)，并提高了纳米纤维膜的热稳定性。在PLGA/CNC复合纳米纤维膜中，负载7 wt.% CNC纳米纤维膜的力学性能最好，与人体皮肤的力学性能相似。细胞培养结果表明，与纯PLGA纳米纤维膜相比，PLGA/CNC复合纳米纤维膜具有更好的细胞相容性，有利于成纤维细胞的粘附、扩散和增殖。这些初步结果表明，PLGA/CNC复合纳米纤维膜在皮肤组织工程领域具有广阔的应用前景。(c) 2015 Elsevier B.V.版权所有
Although extensively used in the fields of drug-carrier and tissue engineering, the biocompatibility and mechanical properties of polylactide-polyglycolide (PLGA) nanofiber membranes still limit their applications. The objective of this study was to improve their utility by introducing cellulose nanocrystals (CNCs) into PLGA nanofiber membranes. PLGA and PLGA/CNC composite nanofiber membranes were prepared via electrospinning, and the morphology and thermodynamic and mechanical properties of these nanofiber membranes were characterized by scanning electron microscopy (SEM), thermogravimetric analysis (TGA), differential scanning calorimetry (DSC), and dynamic mechanical analysis (DMA). The cytocompatibility and cellular responses of the nanofiber membranes were also studied by WST-1 assay, SEM, and confocal laser scanning microscopy (CLSM). Incorporation of CNCs (1, 3, 5, and 7 wt.%) increased the average fiber diameter of the prepared nanofiber membranes from 100 nm (neat PLGA) to similar to 400 nm (PLGA/7 wt.% CNC) and improved the thermal stability of the nanofiber membranes. Among the PLGA/CNC composite nanofiber membranes, those loaded with 7 wt.% CNC nanofiber membranes had the best mechanical properties, which were similar to those of human skin. Cell culture results showed that the PLGA/CNC composite nanofiber membranes had better cytocompatibility and facilitated fibroblast adhesion, spreading, and proliferation compared with neat PLGA nanofiber membranes. These preliminary results suggest that PLGA/CNC composite nanofiber membranes are promising new materials for the field of skin tissue engineering. (c) 2015 Elsevier B.V. All rights reserved.