Characterization of activin A binding sites on the human leukemia cell line K562.
Characterization of activin A binding sites on the human leukemia cell line K562.
复制标题
人白血病细胞系 K562 上激活素 A 结合位点的表征。
DOI：
10.1016/s0006-291x(88)80326-7
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发表时间：
1988
影响因子：
3.1
通讯作者：
Vale,W
中科院分区：
文献类型：
--
作者：
Campen,CA;Vale,W
使用Bolton-Hunter方法将重组激活素A放射性碘标记至高比活性并保持生物活性。人类白血病细胞系K562（已知对激活素A有反应而分化）被发现具有高亲和力[125 I] BH-激活素A受体（Kd <0.13 nM），每个细胞的受体数量较少（每个细胞<600个）。发现该受体具有特异性，因为FSH、LH、GnRH和TGF-β1不竞争结合。这是第一次描述这种蛋白激素在K562细胞上的结合位点。
Recombinant activin A was radioiodinated to a high specific activity with maintenance of bioactivity using the Bolton-Hunter method. The human leukemia cell line K562, known to differentiate in response to activin A, was found to possess high affinity [125I]BH-activin A receptors (Kd∼0.13 nM) with a low number of receptors per cell (∼600 per cell). This receptor was found to be specific as FSH, LH, GnRH, and TGF-β1 do not compete for binding. This is the first description of binding sites for this protein hormone on K562 cells.