Effect of Cell Number on Mesenchymal Stem Cell Transplantation in a Canine Disc Degeneration Model
Effect of Cell Number on Mesenchymal Stem Cell Transplantation in a Canine Disc Degeneration Model
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DOI：
10.1002/jor.21147
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发表时间：
2010-10-01
影响因子：
2.8
通讯作者：
Mochida, Joji
中科院分区：
文献类型：
--
作者：
Serigano, Kenji;Sakai, Daisuke;Mochida, Joji
间充质干细胞（MSC）的移植抑制了动物模型中椎间盘退变的进展我们知道没有研究来确定移植到退变椎间盘（DID）中的细胞的最佳数量为了确定最佳供体细胞数量以获得最大益处，我们使用犬椎间盘退变模型进行了体内研究。将自体MSC以每个椎间盘10（5）、10（6）或10（7）个细胞移植到退行性椎间盘中。使用平片X线摄影、磁共振成像以及大体和显微镜评价对MSC移植的椎间盘进行12周的评价。在MSC-1中可见环状结构，移植组与未移植MSC的手术对照组比较结果移植后剩余的MSC数量，NP细胞的存活率，移植椎间盘中NP细胞的凋亡显示10（6）例MSCs移植椎间盘中维持了结构微环境和丰富的细胞外基质，而在10（5）例移植的MSC中检测到存活细胞较少，而在10（7）例移植的MSC中检测到凋亡细胞较多MSC移植的椎间盘本研究结果表明，移植的细胞数量影响MSC移植物的再生能力，实验性诱导的犬椎间盘退化有人提出，通过从移植细胞和/或驻留细胞产生细胞外基质来维持细胞外基质对于检查导致椎间盘退化的结构破坏的进展是重要的（C）2010年WileyPeriodicals，Inc.出版的OrthopathologyResearchSociety。J Orthop Res 28：1267-1275，2010
Transplantation of mesenchymal stem cells (MSCs) inhibits the progression of disc degeneration in animal models We know of no study to determine the optimal number of cells to transplant Into the degenerated intervertebral disc (DID) To determine the optimal donor cell number for maximum benefit, we conducted an in vivo study using a canine disc degeneration model. Autologous MSCs were transplanted into degenerative discs at 10(5), 10(6), or 10(7) cells per disc The MSC-transplanted discs were evaluated for 12 weeks using plain radiography, magnetic resonance imaging, and gross and microscopic evaluation Preservation of the disc height, annular structure was seen in MSC-transplantation groups compared to the operated control group with no MSC transplantation Result of the number of remaining transplanted MSCs, the survival rate of NP cells, and apoptosis of NP cells in transplanted discs showed both structural microenvironment and abundant extracellular matrix maintained in 10(6) MSCs transplanted disc, while less viable cells were detected in 10(5) MSCs transplanted and more apoptotic cells in 10(7) MSCs transplanted discs The results of this study demonstrate that the number of cells transplanted affects the regenerative capability of MSC transplants in experimentally induced degenerating canine discs It is suggested that maintenance of extracellular matrix by its production from transplanted cells and/or resident cells is Important for checking the progression of structural disruption that leads to disc degeneration (C) 2010 Orthopaedic Research Society Published by Wiley Periodicals, Inc. J Orthop Res 28: 1267-1275, 2010