Exogenous gene integration mediated by genome editing technologies in zebrafish
Exogenous gene integration mediated by genome editing technologies in zebrafish
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DOI：
10.1080/21655979.2017.1300727
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发表时间：
2017-04
期刊：
影响因子：
4.9
通讯作者：
Hitoshi Morita;Kiyohito Taimatsu;Kanoko Yanagi;A. Kawahara
中科院分区：
文献类型：
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作者：
Hitoshi Morita;Kiyohito Taimatsu;Kanoko Yanagi;A. Kawahara
摘要 基因组编辑技术，例如转录激活子样效应核酸酶（TALEN）和成簇规则间隔短回文重复序列（CRISPR）/CRISPR相关蛋白（Cas）系统，可以在目标基因组位点诱导DNA双链断裂（DSB），从而在DSB修复过程中导致移码介导的基因破坏。最近，技术诱导的 DSB 和 DSB 修复被应用于将外源基因整合到各种模式生物的目标基因组位点中。除了由同源定向修复（HDR）介导的传统敲入技术外，还开发了使用精制供体载体的新型敲入技术以及基于其他DSB修复机制的基因组编辑技术，包括非同源末端连接（NHEJ）和微同源介导的末端连接（MMEJ）。因此，改进的敲入技术将有助于自由修改模式生物的基因组。
ABSTRACT Genome editing technologies, such as transcription activator-like effector nuclease (TALEN) and the clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated protein (Cas) systems, can induce DNA double-strand breaks (DSBs) at the targeted genomic locus, leading to frameshift-mediated gene disruption in the process of DSB repair. Recently, the technology-induced DSBs followed by DSB repairs are applied to integrate exogenous genes into the targeted genomic locus in various model organisms. In addition to a conventional knock-in technology mediated by homology-directed repair (HDR), novel knock-in technologies using refined donor vectors have also been developed with the genome editing technologies based on other DSB repair mechanisms, including non-homologous end joining (NHEJ) and microhomology-mediated end joining (MMEJ). Therefore, the improved knock-in technologies would contribute to freely modify the genome of model organisms.