Utp25p, a nucleolar Saccharomyces cerevisiae protein, interacts with U3 snoRNP subunits and affects processing of the 35S pre-rRNA
Utp25p, a nucleolar Saccharomyces cerevisiae protein, interacts with U3 snoRNP subunits and affects processing of the 35S pre-rRNA
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DOI：
10.1111/j.1742-4658.2010.07701.x
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发表时间：
2010-07-01
期刊：
影响因子：
5.4
通讯作者：
Oliveira, Carla C.
中科院分区：
文献类型：
--
作者：
Goldfeder, Mauricio B.;Oliveira, Carla C.
在真核生物中，前rRNA加工依赖于大量的非核糖体反式作用因子，这些因子形成了有趣的组织复合物。两个中间复合物，前40 S和前60 S，在35 S前rRNA加工的早期阶段形成，并产生成熟的核糖体亚基。这些复合物中的每一个都含有特定的前体rRNA、一些核糖体蛋白和加工因子。新的酵母蛋白Utp25p先前已在核仁中被鉴定，这表明该蛋白可能参与核糖体生物合成。在这里，我们表明，Utp25 p与SSU加工体蛋白Sas 10 p和Mpp 10 p相互作用，并影响18S rRNA成熟。Utp25p的缺失导致前rRNA 35S和异常rRNA 23S的积累，以及40S核糖体亚基水平的严重降低。我们的研究结果表明，Utp25 p是一个新的SSU加工体亚基参与前40 S成熟。
In eukaryotes, pre-rRNA processing depends on a large number of nonribosomal trans-acting factors that form intriguingly organized complexes. Two intermediate complexes, pre-40S and pre-60S, are formed at the early stages of 35S pre-rRNA processing and give rise to the mature ribosome subunits. Each of these complexes contains specific pre-rRNAs, some ribosomal proteins and processing factors. The novel yeast protein Utp25p has previously been identified in the nucleolus, an indication that this protein could be involved in ribosome biogenesis. Here we show that Utp25p interacts with the SSU processome proteins Sas10p and Mpp10p, and affects 18S rRNA maturation. Depletion of Utp25p leads to accumulation of the pre-rRNA 35S and the aberrant rRNA 23S, and to a severe reduction in 40S ribosomal subunit levels. Our results indicate that Utp25p is a novel SSU processome subunit involved in pre-40S maturation.