Newly identified RNAs of raspberry leaf blotch virus encoding a related group of proteins
Newly identified RNAs of raspberry leaf blotch virus encoding a related group of proteins
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DOI：
10.1099/jgv.0.000277
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发表时间：
2015-10-01
影响因子：
3.8
通讯作者：
MacFarlane, Stuart
中科院分区：
文献类型：
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作者：
Lu, Yuwen;McGayin, Wendy;MacFarlane, Stuart
Emaravirus属的成员，包括覆盆子叶斑病病毒（RLBV），是具有负链RNA片段基因组的包膜植物病毒，尽管这些病毒的完整基因组补体尚不清楚。目前，小麦花叶病毒拥有最大的伊马拉病毒基因组，包含8个rna。之前，我们鉴定了5种RLBV的基因组rna；在这里，我们确定了另外三个rna （RNA6-8）。RNA6-8编码的蛋白质彼此之间有明确的同源性，但与任何其他肠病毒蛋白质没有同源性。在酵母双杂交和双分子荧光互补（BiFC）实验中，蛋白相互作用，P8蛋白与病毒核衣壳蛋白（P3）通过BiFC相互作用。用马铃薯病毒X表达其中两种蛋白（P6和P7）导致病毒滴度和症状严重程度增加，表明这些蛋白可能在RLBV致病性中起作用；然而，使用两种不同的测试，没有检测到RNA2-8编码的RLBV蛋白的RNA沉默抑制活性。
Members of the genus Emaravirus, including Raspberry leaf blotch virus (RLBV), are enveloped plant viruses with segmented genomes of negative-strand RNA, although the complete genome complement for any of these viruses is not yet clear. Currently, wheat mosaic virus has the largest emaravirus genome comprising eight RNAs. Previously, we identified five genomic RNAs for RLBV; here, we identify a further three RNAs (RNA6-8). RNA6-8 encode proteins that have clear homologies to one another, but not to any other eniaravirus proteins. The proteins self-interacted in yeast two-hybrid and bimolecular fluorescence complementation (BiFC) experiments, and the P8 protein interacted with the virus nucleocapsid protein (P3) using BiFC. Expression of two of the proteins (P6 and P7) using potato virus X led to an increase in virus titre and symptom severity, suggesting that these proteins may play a role in RLBV pathogenicity; however, using two different tests, RNA silencing suppression activity was not detected for any of the RLBV proteins encoded by RNA2-8.