Galangin inhibits epithelial-mesenchymal transition and angiogenesis by downregulating CD44 in glioma
Galangin inhibits epithelial-mesenchymal transition and angiogenesis by downregulating CD44 in glioma
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DOI：
10.7150/jca.31487
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发表时间：
2019-01-01
影响因子：
3.9
通讯作者：
Zheng, Dahai
中科院分区：
文献类型：
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作者：
Chen, Daliang;Li, Dengfeng;Zheng, Dahai
高良姜素（3，5，7-三羟基黄酮）是一种存在于植物中的天然黄酮类化合物，据报道其在包括神经胶质瘤在内的各种类型的癌症中具有抗癌特性。然而，其内在机制尚未完全阐明。CD 44是胶质瘤的标志物，与上皮间质转化（EMT）和血管生成有关，在胶质瘤的发生发展中起重要作用。本研究旨在探讨高良姜素对胶质瘤EMT、血管生成及CD 44表达的抑制作用。高良姜素可抑制胶质瘤细胞的增殖、迁移、侵袭和血管生成，并呈剂量依赖性，通过下调HUVECs中血管内皮生长因子（VEGF）表达，抑制CD 44表达，抑制胶质瘤血管生成。此外，U87和U251细胞中CD 44的过表达部分地取消了高良姜素对胶质瘤细胞的作用。此外，高良姜素抑制颅内胶质瘤小鼠模型中的肿瘤生长。这些结果表明，高良姜素是一种潜在的新型药物，用于胶质母细胞瘤的治疗，由于其能够抑制CD 44，EMT和血管生成。
Galangin (3,5,7-trihydroxyflavone), a natural flavonoid present in plants, has been reported to possess anticancer properties in various types of cancers comprising glioma. The underlying mechanism, however, has not been fully elucidated. CD44, a hall marker in glioma, has been reported to be associated with epithelial-mesenchymal transition (EMT) and angiogenesis, which play important roles in glioma progression. In this study, we aimed to investigate whether galangin can inhibit EMT, angiogenesis and CD44 expression in glioma. We observed that galangin inhibited the proliferation, migration, invasion and angiogenesis of glioma cells in a dose-dependent manner, suppressed the expression of CD44 and inhibited angiogenesis of glioma cells through downregulating vascular endothelial growth factor (VEGF) in HUVECs. In addition, the overexpression of CD44 in U87 and U251 cells partly abolished the effects of galangin on glioma cells. Moreover, galangin suppressed tumor growth in an intracranial glioma mouse model. These results indicate that galangin is a potential novel drug for glioblastoma treatment due to its ability to suppress of CD44, EMT and angiogenesis.