TROSY NMR spectroscopy of large soluble proteins.
TROSY NMR spectroscopy of large soluble proteins.
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DOI：
10.1007/128_2011_228
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发表时间：
2013
影响因子：
8.6
通讯作者：
Yingqi Xu;S. Matthews
中科院分区：
文献类型：
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作者：
Yingqi Xu;S. Matthews
溶液核磁共振光谱通常仅用于研究分子量不超过约50 kDa的蛋白质。近年来，由于标记方法的发展和横向弛豫优化光谱（TROSY）的应用，这种尺寸限制已被显著提升。特别地，甲基特异性标记和甲基-TROSY已被证明对大至约IMDa的超分子系统有效。在这一章中，我们回顾了标记不同种类的甲基和分配策略在非常大的蛋白质系统的可用方法。以几种蛋白质为例，说明NMR如何帮助揭示这些蛋白质的结构、相互作用和动力学的细节。
Solution nuclear magnetic resonance spectroscopy is usually only used to study proteins with molecular weight not exceeding about 50 kDa. This size limit has been lifted significantly in recent years, thanks to the development of labelling methods and the application of transverse-relaxation optimized spectroscopy (TROSY). In particular, methyl-specific labelling and methyl-TROSY have been shown to be effective for supramolecular systems as large as about 1 MDa. In this chapter we review the available methods for labelling different kinds of methyl groups and the assignment strategies in very large protein systems. Several proteins are selected as examples to show how NMR helps to reveal the details of structure, interaction and dynamics of these proteins.