Migrating cells mediate long-range WNT signaling
Migrating cells mediate long-range WNT signaling
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DOI：
10.1242/dev.107656
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发表时间：
2014-05-01
期刊：
影响因子：
4.6
通讯作者：
Marcelle, Christophe
中科院分区：
文献类型：
--
作者：
Serralbo, Olivier;Marcelle, Christophe
在羊膜动物中，人们普遍认为背神经管分泌的 WNT 形成浓度梯度，调节早期体节模式和肌节组织。在这里，我们在鸡胚胎中证明，WNT 蛋白不是被分泌来远距离作用的，而是加载到迁移的神经嵴细胞上，将其传递到体节。抑制神经嵴迁移或消除其数量对体节的 WNT 反应具有深远的影响。此外，我们还发现，硫酸乙酰肝素蛋白聚糖 GPC4 在 WNT 有效递送至体节的过程中发挥着核心作用，由神经嵴表达。总之，我们的数据描述了一种新的信号传导模式，WNT 蛋白可以搭上迁移神经嵴细胞的顺风车，在远离其来源的地方对体节进行模式化。
In amniotes, it is widely accepted that WNTs secreted by the dorsal neural tube form a concentration gradient that regulates early somite patterning and myotome organization. Here we demonstrate in the chicken embryo that WNT protein is not secreted to act at a distance, but rather loaded onto migrating neural crest cells that deliver it to somites. Inhibiting neural crest migration or ablating their population has a profound impact on the WNT response in somites. Furthermore, we show that a central player in the efficient delivery of WNT to somites is the heparan sulfate proteoglycan GPC4, expressed by neural crest. Together, our data describe a novel mode of signaling whereby WNT proteins hitch a ride on migratory neural crest cells to pattern the somites at a distance from its source.