Anaerobic microbial community response to methanogenic inhibitors 2-bromoethanesulfonate and propynoic acid.
Anaerobic microbial community response to methanogenic inhibitors 2-bromoethanesulfonate and propynoic acid.
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DOI：
10.1002/mbo3.349
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发表时间：
2016-08
期刊：
影响因子：
3.4
通讯作者：
Raskin L
中科院分区：
文献类型：
--
作者：
Webster TM;Smith AL;Reddy RR;Pinto AJ;Hayes KF;Raskin L
产甲烷抑制剂常用于研究复杂微生物群落中的产甲烷作用或抑制家畜胃肠道中的产甲烷菌。然而，由此产生的古细菌和细菌群落的结构和功能的变化知之甚少。我们的特点是微生物群落结构和活性的中间生态系统接种牛粪和城市污水处理厂厌氧消化池污泥暴露后，两个产甲烷抑制剂，2-溴乙磺酸盐（BES）和丙炔酸（PA）。甲烷产量分别降低89%（0.5 mmol/L BES）、100%（10 mmol/LBES）、24%（0.1 mmol/LPA）和95%（10 mmol/LPA）。使用针对甲基辅酶M还原酶（mcrA）基因的修饰引物，发现mcrA基因表达的变化与甲烷产量和产甲烷菌相对活性的变化相对应。产甲烷活性由产甲烷菌16 S rRNA cDNA的相对丰度作为总群落16 S rRNA cDNA的百分比来确定。总体而言，产甲烷活性较低时，中围生态系统暴露于较高浓度的两种抑制剂，和aceticarbital产甲烷菌被抑制在更大程度上比氢营养产甲烷菌。通过16 S rRNA cDNA测量的互养细菌活性也在暴露于两种抑制剂后降低，但活性细菌群落的整体结构没有受到显着影响。
Methanogenic inhibitors are often used to study methanogenesis in complex microbial communities or inhibit methanogens in the gastrointestinal tract of livestock. However, the resulting structural and functional changes in archaeal and bacterial communities are poorly understood. We characterized microbial community structure and activity in mesocosms seeded with cow dung and municipal wastewater treatment plant anaerobic digester sludge after exposure to two methanogenic inhibitors, 2‐bromoethanesulfonate (BES) and propynoic acid (PA). Methane production was reduced by 89% (0.5 mmol/L BES), 100% (10 mmol/LBES), 24% (0.1 mmol/LPA), and 95% (10 mmol/LPA). Using modified primers targeting the methyl‐coenzyme M reductase (mcrA) gene, changes in mcrA gene expression were found to correspond with changes in methane production and the relative activity of methanogens. Methanogenic activity was determined by the relative abundance of methanogen 16S rRNA cDNA as a percentage of the total community 16S rRNA cDNA. Overall, methanogenic activity was lower when mesocosms were exposed to higher concentrations of both inhibitors, and aceticlastic methanogens were inhibited to a greater extent than hydrogenotrophic methanogens. Syntrophic bacterial activity, measured by 16S rRNA cDNA, was also reduced following exposure to both inhibitors, but the overall structure of the active bacterial community was not significantly affected.