TIPPING AND MATING-STRUCTURE ACTIVATION INDUCED IN CHLAMYDOMONAS GAMETES BY FLAGELLAR MEMBRANE ANTISERA
TIPPING AND MATING-STRUCTURE ACTIVATION INDUCED IN CHLAMYDOMONAS GAMETES BY FLAGELLAR MEMBRANE ANTISERA
复制标题
DOI：
10.1083/jcb.79.3.680
复制
发表时间：
1978-01-01
影响因子：
7.8
通讯作者：
JURIVICH, D
中科院分区：
文献类型：
--
作者：
GOODENOUGH, UW;JURIVICH, D
抗C. Reinhardi已被用于探测鞭毛膜的动态特性。通过鞭毛凝集细胞的抗血清与营养膜和配子膜以及两种交配型（mt+和mt-）膜上存在的抗原结合。配子细胞对抗体呈递的反应与营养细胞非常不同，甚至可以动员高浓度的抗体向鞭毛尖端;讨论了这种“倾斜”能力反映了与性凝集性相关的分化配子特性的可能性。配子细胞也通过激活它们的交配结构对抗体凝集反应，mt+反应涉及微丝的快速聚合。几种不能进行性凝集的无能mt+突变株也被抗血清激活，并与正常mt-配子形成合子。相似交配类型的激活细胞之间不发生融合。抗体的单价（Fab）制剂不能激活mt+配子，这表明抗血清的交联特性对于其模拟或绕过性凝集的能力是必不可少的。
Antisera raised against vegetative and gametic flagella of C. reinhardi have been used to probe dynamic properties of the flagellar membranes. The antisera, which agglutinate cells via their flagella, associate with antigens that are present on both vegetative and gametic membranes and on membranes of both mating types (mt+ and mt-). Gametic cells respond to antibody presentation very differently from vegetative cells, mobilizing even high concentrations of antibody towards the flagellar tips; the possibility is discussed that such "tipping" ability reflects a differentiated gametic property relevant to sexual agglutinability. Gametic cells also respond to antibody agglutination by activating their mating structures, the mt+ reaction involving a rapid polymerization of microfilaments. Several impotent mt+ mutant strains that fail to agglutinate sexually are also activated by the antisera and proceed to form zygotes with normal mt- gametes. Fusion does not occur between activated cells of like mating type. Monovalent (Fab) preparations of the antibody fail to activate mt+ gametes, suggesting that the cross-linking properties of the antisera are essential for their ability to mimic, or bypass, sexual agglutination.